Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae

The internalization step of endocytosis in yeast requires actin and sterols for maximum efficiency. In addition, many receptors and plasma membrane proteins must be phosphorylated and ubiquitylated prior to internalization. The Saccharomyces cerevisiae end8‐1 mutant is allelic to lcb1, a mutant defe...

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Bibliographic Details
Published in:The EMBO journal 2000-06, Vol.19 (12), p.2824-2833
Main Authors: Zanolari, Bettina, Friant, Sylvie, Funato, Kouichi, Sütterlin, Christine, Stevenson, Brian J, Riezman, Howard
Format: Article
Language:English
Subjects:
actin
Acyltransferases
Acyltransferases - genetics
Acyltransferases - metabolism
Cellular Biology
ceramide
Ceramides
Ceramides - biosynthesis
Endocytosis
Endocytosis - physiology
lcb1
Life Sciences
Mutation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins
Serine C-Palmitoyltransferase
sphingoid base
sphingoid bases
Sphingolipids
Sphingolipids - biosynthesis
Sphingosine
Sphingosine - analogs & derivatives
Sphingosine - biosynthesis
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Summary:The internalization step of endocytosis in yeast requires actin and sterols for maximum efficiency. In addition, many receptors and plasma membrane proteins must be phosphorylated and ubiquitylated prior to internalization. The Saccharomyces cerevisiae end8‐1 mutant is allelic to lcb1, a mutant defective in the first step of sphingoid base synthesis. Upon arrest of sphingoid base synthesis a rapid block in endocytosis is seen. This block can be overcome by exogenous sphingoid base. Under conditions where endogenous sphingosine base synthesis was blocked and exogenous sphingoid bases could not be converted to phosphorylated sphingoid bases or to ceramide, sphingoid bases could still suppress the endocytic defect. Therefore, the required lipid is most likely a sphingoid base. Interestingly, sphingoid base synthesis is required for proper actin organization, but is not required for receptor phosphorylation. This is the first case of a physiological role for sphingoid base synthesis, other than as a precursor for ceramide or phosphorylated sphingoid base synthesis.
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1093/emboj/19.12.2824