Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response

SUMMARY Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in...

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Bibliographic Details
Published in:Clinical and experimental immunology 2002-02, Vol.127 (2), p.206-213
Main Authors: MOSER, C., JENSEN, P. O., KOBAYASHI, O., HOUGEN, H. P., SONG, Z., RYGAARD, J., KHARAZMI, A., H?BY, N.
Format: Article
Language:English
Subjects:
Agar
Alginates
Animal Studies
Animals
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - blood
Bacterial diseases
Bacterial diseases of the respiratory system
Biological and medical sciences
Bronchoalveolar Lavage Fluid
CD4 Lymphocyte Count
Chronic Disease
cytokines
Cytokines - biosynthesis
Female
Glucuronic Acid
Hexuronic Acids
Human bacterial diseases
Immunoglobulin G - biosynthesis
Immunoglobulin G - blood
Infectious diseases
infectious immunity‐bacteria
Interferon-gamma - biosynthesis
Interleukin-12 - biosynthesis
Interleukin-4 - biosynthesis
lung
Lymphocyte Function-Associated Antigen-1 - biosynthesis
Medical sciences
Mice
Mice, Inbred BALB C
Models, Animal
Pneumonia, Bacterial - immunology
Pseudomonas aeruginosa
Pseudomonas Infections - immunology
Recurrence
rodent
Th1 Cells - immunology
Th1 Cells - metabolism
Th1/Th2
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Summary:SUMMARY Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible BALB/c mice were re‐infected with P. aeruginosa 14 days after the initial infection. Singly‐infected BALB/c mice, as well as non‐infected mice, were used as controls. Decreased mortality and milder lung inflammation in re‐infected BALB/c mice, as well as a tendency for improved clearance of bacteria, was observed when compared with singly‐infected mice. The improved outcome in re‐infected mice correlated with changes in CD4 cell numbers. Surface expression of LFA‐1 on pulmonary CD4 cells was increased in re‐infected compared with singly‐infected mice. Moreover, resistance to re‐infection was paralleled by a shift towards a Th1‐dominated response and increased IL‐12 production. No significant increase in serum IgG was observed in the re‐infected mice. In conclusion, these results indicate a protective role for a Th1‐dominated response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.2002.01731.x