CD8+T cell‐mediated enhancement of tumour necrosis factor‐alpha (TNF‐α) production and HIV‐1 LTR‐driven gene expression in human monocytic cells is pertussis toxin‐sensitive

HIV replication and LTR‐mediated gene expression can be modulated by CD8+T cells in a cell type‐dependent manner. We have previously shown that supernatants of activated CD8+ T cells of HIV‐infected individuals greatly enhanced p24 levels in human macrophages infected with NSI or SI primary isolates...

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Bibliographic Details
Published in:Clinical and experimental immunology 1999-06, Vol.116 (3), p.479-485
Main Authors: COPELAND, K. F. T, MCKAY, P. J, NEWTON, J, ROSENTHAL, K. L
Format: Article
Language:English
Subjects:
AIDS/HIV
Biological and medical sciences
CD8+ T cell
CD8-Positive T-Lymphocytes - drug effects
CD8-Positive T-Lymphocytes - immunology
Cell Line
Chloramphenicol O-Acetyltransferase - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression - drug effects
Genes, Reporter - drug effects
HIV
HIV Infections - immunology
HIV Infections - virology
HIV Long Terminal Repeat - drug effects
HIV-1 - drug effects
HIV-1 - genetics
HIV-1 - physiology
Humans
macrophage
Microbiology
Monocytes - drug effects
Monocytes - immunology
Monocytes - virology
Original
Pertussis Toxin
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
transcription
Tumor Necrosis Factor-alpha - biosynthesis
Virology
Virulence Factors, Bordetella - pharmacology
Virus Replication - drug effects
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Summary:HIV replication and LTR‐mediated gene expression can be modulated by CD8+T cells in a cell type‐dependent manner. We have previously shown that supernatants of activated CD8+ T cells of HIV‐infected individuals greatly enhanced p24 levels in human macrophages infected with NSI or SI primary isolates of HIV‐1. Here we have examined the effect of culture with CD8+T cell supernatants on HIV‐1 LTR‐mediated gene expression in monocytic cells. CD8+T cell supernatants enhanced LTR‐mediated gene expression in U38 cells activated with Tat in the absence or presence of phorbol myristate acetate (PMA) and ionomycin or TNF‐α. Further, enhancement of LTR‐mediated gene expression and virus replication in U38 cells and U1 cells, respectively, was pertussis toxin‐sensitive. The enhancement of gene expression and virus replication was associated with increased levels of TNF‐α and was significantly abrogated by antibody to TNF‐α. In contrast, the suppression of LTR‐mediated gene expression by CD8+T cell supernatants in Jurkat T cells was not pertussis toxin‐sensitive and TNF‐α levels were not affected. These results demonstrate that factors produced by CD8+T cells utilize different cellular pathways to mediate their effects on HIV transcription and replication in different cell types.
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.1999.00897.x