Catalase and α‐enolase: two novel granulocyte autoantigens in inflammatory bowel disease (IBD)

In IBD, the target antigens of anti‐neutrophil cytoplasmic autoantibodies (ANCA) have not been fully identified, which limits the analysis of the diagnostic significance as well as of the possible pathophysiological role of these antibodies. In this study, we identify the target antigens of ANCA in...

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Published in:	Clinical and experimental immunology 1998-04, Vol.112 (1), p.10-16
Main Authors:	ROOZENDAAL, C, ZHAO, M. H, HORST, G, LOCKWOOD, C. M, KLEIBEUKER, J. H, LIMBURG, P. C, NELIS, G. F, KALLENBERG, C. G
Format:	Article
Language:	eng
Subjects:	Antibodies, Antineutrophil Cytoplasmic - immunology anti‐neutrophil cytoplasmic antibodies Autoantigens - immunology Biological and medical sciences catalase Catalase - blood Catalase - immunology Gastroenterology. Liver. Pancreas. Abdomen Granulocytes - immunology Humans inflammatory bowel disease Inflammatory Bowel Diseases - blood Inflammatory Bowel Diseases - immunology Medical sciences Original Other diseases. Semiology Phosphopyruvate Hydratase - blood Phosphopyruvate Hydratase - immunology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus α‐enolase
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Summary:	In IBD, the target antigens of anti‐neutrophil cytoplasmic autoantibodies (ANCA) have not been fully identified, which limits the analysis of the diagnostic significance as well as of the possible pathophysiological role of these antibodies. In this study, we identify the target antigens of ANCA in large groups of patients with ulcerative colitis (UC) and Crohn's disease (CD). Apart from antibodies against lactoferrin and bactericidal/permeability‐increasing protein (BPI), which have been reported before, antibodies against two novel granulocyte antigens were identified: antibodies against a 57/56‐kD doublet were found in 38% of samples from UC patients and in 26% of samples from CD patients, whereas antibodies against a 47‐kD protein were found in 10% of samples from UC patients and in 18% of samples from CD patients. Partial purification and amino acid sequence analysis identified the 57‐kD protein as catalase and the 47‐kD protein as α‐enolase. This study is the first to report catalase and α‐enolase as granulocyte antigens for autoantibodies in IBD.
ISSN:	0009-9104 1365-2249