EGO, a novel, noncoding RNA gene, regulates eosinophil granule protein transcript expression

Gene expression profiling of early eosinophil development shows increased transcript levels of proinflammatory cytokines, chemokines, transcription factors, and a novel gene, EGO (eosinophil granule ontogeny). EGO is nested within an intron of the inositol triphosphate receptor type 1 (ITPR1) gene a...

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Bibliographic Details
Published in:Blood 2007-06, Vol.109 (12), p.5191-5198
Main Authors: Wagner, Lori A., Christensen, Clarissa J., Dunn, Diane M., Spangrude, Gerald J., Georgelas, Ann, Kelley, Linda, Esplin, M. Sean, Weiss, Robert B., Gleich, Gerald J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cells, Cultured
Eosinophil Granule Proteins - genetics
Eosinophil Major Basic Protein - genetics
Eosinophil-Derived Neurotoxin - genetics
Eosinophils - metabolism
Gene Expression Profiling
Gene Expression Regulation - genetics
Hematologic and hematopoietic diseases
Hematopoiesis
Hematopoietic Stem Cells - metabolism
Humans
Inositol 1,4,5-Trisphosphate Receptors - genetics
Medical sciences
RNA, Untranslated - genetics
RNA, Untranslated - physiology
Transcription, Genetic
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Summary:Gene expression profiling of early eosinophil development shows increased transcript levels of proinflammatory cytokines, chemokines, transcription factors, and a novel gene, EGO (eosinophil granule ontogeny). EGO is nested within an intron of the inositol triphosphate receptor type 1 (ITPR1) gene and is conserved at the nucleotide level; however, the largest open reading frame (ORF) is 86 amino acids. Sucrose density gradients show that EGO is not associated with ribosomes and therefore is a noncoding RNA (ncRNA). EGO transcript levels rapidly increase following interleukin-5 (IL-5) stimulation of CD34+ hematopoietic progenitors. EGO RNA also is highly expressed in human bone marrow and in mature eosinophils. RNA silencing of EGO results in decreased major basic protein (MBP) and eosinophil derived neurotoxin (EDN) mRNA expression in developing CD34+ hematopoietic progenitors in vitro and in a CD34+ cell line model. Therefore, EGO is a novel ncRNA gene expressed during eosinophil development and is necessary for normal MBP and EDN transcript expression.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2006-06-027987