Promoter proximal splice sites enhance transcription

Reconstruction of a gene with its introns removed results in reduced levels of cytoplasmic mRNA. This is partly explained by introns promoting the export of mRNA through coupling splicing to nuclear export processes. However, we show here that splicing signals can have a direct role in enhancing gen...

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Bibliographic Details
Published in:Genes & development 2002-11, Vol.16 (21), p.2792-2799
Main Authors: Furger, Andre, O'Sullivan, Justin M, Binnie, Alexandra, Lee, Barbara A, Proudfoot, Nick J
Format: Article
Language:English
Subjects:
Genes, Fungal
Genes, Viral
HIV-1 - genetics
Promoter Regions, Genetic - genetics
Research Paper
RNA Splice Sites - genetics
RNA, Messenger - genetics
Saccharomyces cerevisiae - genetics
Transcription, Genetic
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Summary:Reconstruction of a gene with its introns removed results in reduced levels of cytoplasmic mRNA. This is partly explained by introns promoting the export of mRNA through coupling splicing to nuclear export processes. However, we show here that splicing signals can have a direct role in enhancing gene transcription. Removal of promoter proximal splice signals from a mammalian gene or the excision of introns from two different yeast genes results in a marked reduction in levels of nascent transcription, based on both nuclear run-on and direct image analysis. This further establishes that mRNA processing and transcription are tightly coupled mechanisms.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.983602