Acute distal colitis impairs gastric emptying in rats via an extrinsic neuronal reflex pathway involving the pelvic nerve

Background and aims: Patients with inflammatory bowel disease often present with abnormal gut motility away from the inflammatory site. We studied remote motility disturbances and their pathophysiology in a rat model of colitis. Methods: Colitis was induced 72 h prior to experiments using trinitrobe...

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Bibliographic Details
Published in:Gut 2007-02, Vol.56 (2), p.195-202
Main Authors: De Schepper, H U, De Man, J G, Van Nassauw, L, Timmermans, J-P, Herman, A G, Pelckmans, P A, De Winter, B Y
Format: Article
Language:English
Subjects:
Acute Disease
Analgesics, Non-Narcotic - pharmacology
analysis of variance
Animals
ANOVA
Biological and medical sciences
calcitonin gene related peptide
Capsaicin - pharmacology
CGRP
Colitis - chemically induced
Colitis - pathology
Colitis - physiopathology
Colon
dorsal root ganglion
DRG
Ganglia, Spinal - physiopathology
Ganglionic Blockers - pharmacology
Gastric Emptying - physiology
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal Motility - physiology
Gastrointestinal Transit - physiology
Gastroparesis - pathology
Gastroparesis - physiopathology
GCI
GCS+I
geometric centre of intestine
geometric centre of stomach and intestine
Hexamethonium - pharmacology
IBD
Immunohistochemistry - methods
Inflammatory bowel disease
Intestinal Mucosa - pathology
Intestinal Mucosa - physiopathology
Male
Medical sciences
MPO
myeloperoxidase
Neural Pathways - physiology
Neurogastroenterology
Neurons - physiology
Other diseases. Semiology
PBS
Pelvis - innervation
Peroxidase - metabolism
phosphate buffered saline
Proto-Oncogene Proteins c-fos - analysis
Rats
Rats, Wistar
Rodents
Small intestine
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
TNBS
trinitrobenzene sulphate
Trinitrobenzenesulfonic Acid
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Summary:Background and aims: Patients with inflammatory bowel disease often present with abnormal gut motility away from the inflammatory site. We studied remote motility disturbances and their pathophysiology in a rat model of colitis. Methods: Colitis was induced 72 h prior to experiments using trinitrobenzene sulphate (TNBS) instillation. Inflammation was verified using histology and myeloperoxidase (MPO) measurements. To assess gut motility, we determined gastric emptying, distal front and geometric centre (GC) of intestinal transit 30 min after intragastric administration of a semiliquid Evans blue solution. The effects of hexamethonium (20 mg/kg), capsaicin (125 mg/kg) and pelvic nerve section on colitis induced motility changes were evaluated. c-Fos expression was studied in the pelvic nerve dorsal root ganglion (DRG) S1. Results: Colitis reduced gastric emptying from 38.4 (3.6)% in controls to 22.7 (4.4)% in TNBS treated rats in the absence of local gastric inflammation. Colitis had no effect on the distal front or on the geometric centre of small intestinal transit. Hexamethonium reduced gastric emptying in controls to 26.3 (4.1)% but restored it to 35.8 (4.4)% in TNBS treated rats. Capsaicin significantly impaired gastric emptying in controls from 33.1 (5.2)% to 9.5 (3.3)% while this effect was less pronounced in TNBS treated rats (from 19.2 (2.3)% to 11.5 (3.8)%; NS). In TNBS treated rats, pelvic nerve section completely restored gastric emptying from 19.8 (5.3)% to 52.5 (6.3)% without any effect on gastric emptying in control rats. TNBS colitis induced de novo c-Fos expression in the DRG S1. Conclusions: Experimental colitis in rats delays gastric emptying via a neuronal pathway involving pelvic afferent nerve hyperactivity.
ISSN:0017-5749
1468-3288
DOI:10.1136/gut.2006.104745