Interactions among PIN-FORMED and P-Glycoprotein Auxin Transporters in Arabidopsis

Directional transport of the phytohormone auxin is established primarily at the point of cellular efflux and is required for the establishment and maintenance of plant polarity. Studies in whole plants and heterologous systems indicate that PIN-FORMED (PIN) and P-glycoprotein (PGP) transport protein...

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Published in:The Plant cell 2007-01, Vol.19 (1), p.131-147
Main Authors: Blakeslee, Joshua J, Bandyopadhyay, Anindita, Lee, Ok Ran, Mravec, Jozef, Titapiwatanakun, Boosaree, Sauer, Michael, Makam, Srinivas N, Cheng, Yan, Bouchard, Rodolphe, Adamec, Jiří, Geisler, Markus, Nagashima, Akitomo, Sakai, Tatsuya, Martinoia, Enrico, Friml, Jiří, Peer, Wendy Ann, Murphy, Angus S
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - analysis
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
ATP Binding Cassette Transporter, Subfamily B - analysis
ATP Binding Cassette Transporter, Subfamily B - genetics
ATP Binding Cassette Transporter, Subfamily B - metabolism
ATP-Binding Cassette Transporters - analysis
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
ATP-Binding Cassette Transporters - physiology
Auxins
Biological Transport - physiology
Cell membranes
HeLa cells
Hypocotyl - genetics
Hypocotyl - metabolism
Hypocotyls
Indoleacetic Acids - metabolism
Membrane Transport Proteins - analysis
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Mutation
Plant cells
Plant roots
Plant Roots - genetics
Plant Roots - metabolism
Plants
Proteins
RNA, Messenger - metabolism
Seedlings
Two-Hybrid System Techniques
Yeasts
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Summary:Directional transport of the phytohormone auxin is established primarily at the point of cellular efflux and is required for the establishment and maintenance of plant polarity. Studies in whole plants and heterologous systems indicate that PIN-FORMED (PIN) and P-glycoprotein (PGP) transport proteins mediate the cellular efflux of natural and synthetic auxins. However, aromatic anion transport resulting from PGP and PIN expression in nonplant systems was also found to lack the high level of substrate specificity seen in planta. Furthermore, previous reports that PGP19 stabilizes PIN1 on the plasma membrane suggested that PIN-PGP interactions might regulate polar auxin efflux. Here, we show that PGP1 and PGP19 colocalized with PIN1 in the shoot apex in Arabidopsis thaliana and with PIN1 and PIN2 in root tissues. Specific PGP-PIN interactions were seen in yeast two-hybrid and coimmunoprecipitation assays. PIN-PGP interactions appeared to enhance transport activity and, to a greater extent, substrate/inhibitor specificities when coexpressed in heterologous systems. By contrast, no interactions between PGPs and the AUXIN1 influx carrier were observed. Phenotypes of pin and pgp mutants suggest discrete functional roles in auxin transport, but pin pgp mutants exhibited phenotypes that are both additive and synergistic. These results suggest that PINs and PGPs characterize coordinated, independent auxin transport mechanisms but also function interactively in a tissue-specific manner.
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1105/tpc.106.040782