Stimulation of toll‐like receptor 2 in mononuclear cells from HIV‐infected patients induces chemokine responses: possible pathogenic consequences

SUMMARY Toll‐like receptor 2 (TLR2) stimulation in monocytes may contribute to enhanced inflammation and viral replication in HIV infection. In the present study we examined if TLR2 stimulation could modulate chemokine responses in peripheral blood mononuclear cells (PBMC) from HIV‐infected patients...

Full description

Saved in:

Bibliographic Details
Published in:	Clinical and experimental immunology 2004-10, Vol.138 (1), p.116-121
Main Authors:	HEGGELUND, L., DAMÅS, J. K., YNDESTAD, A., HOLM, A. M., MÜLLER, F., LIEN, E., ESPEVIK, T., AUKRUST, P., FRØLAND, S. S.
Format:	Article
Language:	eng
Subjects:	Adult Antiretroviral Therapy, Highly Active - methods Biological and medical sciences Chemokine CCL2 - immunology Chemokine CCL3 Chemokine CCL4 Chemokine CCL5 - analysis chemokines Chemokines - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology HIV Infections - drug therapy HIV Infections - immunology HIV/AIDS Human immunodeficiency virus Humans Immunopathology innate immunity Interferon-gamma - immunology Interleukin-8 - analysis Interleukin-8 - immunology Leukocytes, Mononuclear - immunology Macrophage Inflammatory Proteins - analysis Macrophage Inflammatory Proteins - immunology Male Medical sciences Membrane Glycoproteins - immunology Middle Aged monocytes Original Receptors, CCR5 - analysis Receptors, Cell Surface - immunology Recombinant Proteins - immunology RNA, Messenger - analysis RNA, Viral - analysis Toll-Like Receptor 2 Toll-Like Receptors Up-Regulation - immunology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	SUMMARY Toll‐like receptor 2 (TLR2) stimulation in monocytes may contribute to enhanced inflammation and viral replication in HIV infection. In the present study we examined if TLR2 stimulation could modulate chemokine responses in peripheral blood mononuclear cells (PBMC) from HIV‐infected patients and healthy controls. Our main findings were, with similar qualitative patterns in both healthy controls and HIV‐infected patients: (1) TLR2 stimulation induced up‐regulation of several chemokines at the mRNA level as well as increased protein levels of macrophage inflammatory protein (MIP)‐1α, interleukin (IL)‐8 and regulated on activation, normal T cell expressed and secreted (RANTES); (2) TLR2 stimulation induced enhanced protein expression of CCR5 (a receptor for MIP‐1α and RANTES) on monocytes; (3) In vitro stimulation with RANTES induced release of MIP‐1α, MCP‐1, IL‐8 and interferon‐γ from PBMC. While increased levels of β‐chemokines possibly have antiviral effects, TLR2 stimulation may also promote a chemokine‐driven inflammatory loop, potentially contributing to the immunopathogenesis of HIV infection.
ISSN:	0009-9104 1365-2249