Anti‐Saccharomyces cerevisiae antibodies (ASCA) in Crohn's disease are associated with disease severity but not NOD2/CARD15 mutations

SUMMARY Anti‐Saccharomyces cerevisiae antibodies (ASCAs) have been proposed as serological markers, which may differentiate Crohn's disease (CD) from ulcerative colitis (UC) and predict disease phenotype. Their importance in pathogenesis is unproven. We investigated the relationship between ASC...

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Bibliographic Details
Published in:Clinical and experimental immunology 2004-03, Vol.135 (3), p.490-496
Main Authors: WALKER, L. J., ALDHOUS, M. C., DRUMMOND, H. E., SMITH, B. R. K., NIMMO, E. R., ARNOTT, I. D. R., SATSANGI, J.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age of Onset
Aged
Aged, 80 and over
Antibodies, Fungal - biosynthesis
Antibodies, Fungal - blood
ASCA
Biological and medical sciences
Biomarkers - blood
CARD15
Carrier Proteins - genetics
Clinical Studies
Colitis, Ulcerative - immunology
Crohn Disease - genetics
Crohn Disease - immunology
Crohn Disease - pathology
Crohn's disease
disease behaviour
disease location
Disease Progression
Female
Follow-Up Studies
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genotype
Humans
Immunoglobulin A - biosynthesis
Immunoglobulin G - biosynthesis
Immunopathology
Intracellular Signaling Peptides and Proteins
Male
Medical sciences
Middle Aged
Mutation
NOD2
Nod2 Signaling Adaptor Protein
Phenotype
Saccharomyces cerevisiae
Saccharomyces cerevisiae - immunology
Sensitivity and Specificity
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Summary:SUMMARY Anti‐Saccharomyces cerevisiae antibodies (ASCAs) have been proposed as serological markers, which may differentiate Crohn's disease (CD) from ulcerative colitis (UC) and predict disease phenotype. Their importance in pathogenesis is unproven. We investigated the relationship between ASCAs, disease phenotype and NOD2/CARD15 genotype in CD and whether ASCAs were related to antibodies to other fungal proteins. Serum from 228 patients [143 CD, 75 UC, 10 with indeterminate colitis (IC)] and 78 healthy controls (HC) were assayed for ASCA. Antibodies (IgA, IgG) to other fungal proteins (Fusarium species ATC20334, Mycoprotein) were measured in the same samples using an in‐house enzyme‐linked immunosorbent assay (ELISA) assay. ASCAs were present in 57% of CD, 19% of UC, 30% of IC and 8% of HCs. ASCA‐positive status was a predictor for CD with sensitivity of 57%, specificity of 87%, positive predictive value of 78% and negative predictive value of 68%. ASCA was associated with proximal (gastroduodenal and small bowel involvement) rather than purely colonic disease (P 
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2003.02392.x