VAMP8/endobrevin as a general vesicular SNARE for regulated exocytosis of the exocrine system

The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be the major vesicular SNARE (v-SNARE) of zymogen granules of pancreatic exocrine acinar cells. In this article, we have characterized the role of...

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Bibliographic Details
Published in:Molecular biology of the cell 2007-03, Vol.18 (3), p.1056-1063
Main Authors: Wang, Cheng-Chun, Shi, Hong, Guo, Ke, Ng, Chee Peng, Li, Jie, Gan, Bin Qi, Chien Liew, Hwee, Leinonen, Jukka, Rajaniemi, Hannu, Zhou, Zhi Hong, Zeng, Qi, Hong, Wanjin
Format: Article
Language:English
Subjects:
Animals
Exocrine Glands - cytology
Exocrine Glands - metabolism
Exocytosis
Lacrimal Apparatus - cytology
Lacrimal Apparatus - ultrastructure
Male
Mice
Protein Structure, Quaternary
Proteins - chemistry
R-SNARE Proteins - deficiency
R-SNARE Proteins - metabolism
Salivary Glands - cytology
Salivary Glands - metabolism
Salivary Glands - ultrastructure
Secretory Vesicles - ultrastructure
SNARE Proteins - metabolism
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Summary:The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be the major vesicular SNARE (v-SNARE) of zymogen granules of pancreatic exocrine acinar cells. In this article, we have characterized the role of VAMP8 in the entire exocrine system. Immunohistochemical studies showed that VAMP8 is expressed in all examined exocrine tissues such as salivary glands, lacrimal (tear) glands, sweat glands, sebaceous glands, mammary glands, and the prostate. Severe anomalies were observed in the salivary and lacrimal glands of VAMP8-null mice. Mutant salivary glands accumulated amylase and carbonic anhydrase VI. Electron microscopy revealed an accumulation of secretory granules in the acinar cells of mutant parotid and lacrimal glands. Pilocarpine-stimulated secretion of saliva proteins was compromised in the absence of VAMP8. Protein aggregates were observed in mutant lacrimal glands. VAMP8 may interact with syntaxin 4 and SNAP-23. These results suggest that VAMP8 may act as a v-SNARE for regulated secretion of the entire exocrine system.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e06-10-0974