Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid

Background: Trinitrobenzene sulphonic acid (TNBS) induces chronic transmural inflammatory lesions in the rat colon. Injury is facilitated by barrier disruption and invasion of commensal bacteria. However, certain bacteria have shown anti-inflammatory properties in in vitro models. Aim: To investigat...

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Bibliographic Details
Published in:Gut 2005-07, Vol.54 (7), p.955-959
Main Authors: Llopis, M, Antolín, M, Guarner, F, Salas, A, Malagelada, J-R
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Bacteria
Bacterial Translocation
CFU
colitis
Colitis, Ulcerative - chemically induced
Colitis, Ulcerative - microbiology
Colitis, Ulcerative - therapy
Colon - microbiology
colony forming units
De Man Rogosa Sharp medium
Disease Models, Animal
Experiments
Gangrene
Inflammation
Inflammatory Bowel Disease
Intestinal Absorption
Intestinal Mucosa - microbiology
Laboratory animals
lactobacillus
Lactobacillus casei
Lactobacillus casei - physiology
Liver - microbiology
Lymph Nodes - microbiology
Male
mesenteric lymph nodes
MLN
MPO
MRS
mucosal barrier trinitrobenzene sulphonic acid
myeloperoxidase
Peroxidase - metabolism
probiotics
Probiotics - therapeutic use
Rats
Rats, Sprague-Dawley
Rodents
Small intestine
Spleen - microbiology
Studies
Surgery
TNBS
trinitrobenzene sulphonic acid
Trinitrobenzenesulfonic Acid
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Summary:Background: Trinitrobenzene sulphonic acid (TNBS) induces chronic transmural inflammatory lesions in the rat colon. Injury is facilitated by barrier disruption and invasion of commensal bacteria. However, certain bacteria have shown anti-inflammatory properties in in vitro models. Aim: To investigate in vivo the anti-inflammatory effect of Lactobacillus casei DN-114 001. Methods: Rats with a colonic segment excluded from faecal transit were surgically prepared. After washing the lumen with antibiotics, the excluded segment was recolonised (control group: standard flora of rat origin; test group: standard flora and L casei). Microbial colonisation was confirmed by culture of segment washing, and colitis was then induced by instillation of TNBS. One day after, intestinal lesions were blindly graded by macro- and microscopic scores, and myeloperoxidase activity measured in tissue homogenates. Translocation of bacteria to mesenteric lymph nodes, spleen and liver was investigated. Results: Test rats showed a smaller area of mucosal injury than control rats (p
ISSN:0017-5749
1468-3288
1458-3288
DOI:10.1136/gut.2004.056101