DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture

Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with...

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Bibliographic Details
Published in:British journal of ophthalmology 2002-11, Vol.86 (11), p.1303-1305
Main Authors: Eter, N, Spitznas, M
Format: Article
Language:English
Subjects:
Angiogenesis Inhibitors - pharmacology
Animals
Biological and medical sciences
Capillaries - cytology
Capillaries - drug effects
Cattle
Cell culture
Cell Division - drug effects
Cell growth
cell proliferation
Cells, Cultured
choriocapillary endothelial cells
Choroid - blood supply
Choroid - cytology
Choroid - drug effects
Dimethyl Sulfoxide - pharmacology
DMSO
Endothelium - cytology
Endothelium - drug effects
Experiments
Eye
Immunoenzyme Techniques
Laboratory Science
Macular degeneration
Medical sciences
Microscopy, Fluorescence
Pharmacology. Drug treatments
Solvents
Statistical analysis
thalidomide
Thalidomide - pharmacology
Vascular endothelial growth factor
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Summary:Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.
ISSN:0007-1161
1468-2079
DOI:10.1136/bjo.86.11.1303