Immunodetection of SV40 large T antigen in human central nervous system tumours

Background/Aims: DNA sequences from Simian virus 40 (SV40) have been previously isolated from various human tumours of the central nervous system (CNS). This study aimed to investigate a series of tumours of the CNS for the expression of the SV40 large T antigen (Tag), which is an oncogenic protein...

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Bibliographic Details
Published in:Journal of clinical pathology 2005-04, Vol.58 (4), p.429-431
Main Authors: Sabatier, J, Uro-Coste, E, Benouaich, A, Boetto, S, Gigaud, M, Tremoulet, M, Delisle, M-B, Galateau-Sallé, F, Brousset, P
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antigens, Polyomavirus Transforming - analysis
catalysed system amplification
central nervous system
Central Nervous System Neoplasms - immunology
Central Nervous System Neoplasms - virology
Child
Child, Preschool
CNS
CSA
Humans
immunohistochemistry
Immunohistochemistry - methods
Middle Aged
PCR
polymerase chain reaction
Short Reports/Case Reports
Simian virus 40
Simian virus 40 large T antigen
SV40
T antigen
Tag
tumours
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Summary:Background/Aims: DNA sequences from Simian virus 40 (SV40) have been previously isolated from various human tumours of the central nervous system (CNS). This study aimed to investigate a series of tumours of the CNS for the expression of the SV40 large T antigen (Tag), which is an oncogenic protein of the virus. Methods: A French series of 82 CNS tumours was investigated for Tag expression using a monoclonal antibody and immunohistochemistry. A Tag positive hepatocellular carcinoma cell line from transgenic mice and a kidney biopsy from a patient infected by SV40 were used as positive controls. Results: None of the tumours (20 ependymomas, 20 glioblastomas, 12 oligodendrogliomas, three plexus choroid adenomas, two plexus choroid carcinomas, 15 meningiomas, and 10 medulloblastomas) contained SV40 Tag positive cells. Conclusions: The lack of SV40 Tag in 82 CNS tumours of various types is at variance with previous studies from different countries, and suggests that the virus may not be an important factor in CNS tumorigenesis, at least in French cases.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.2004.020131