Association between TNF-α promoter polymorphism and Helicobacter pylori cagA subtype infection

Aims—To assess the importance of tumour necrosis factor α (TNF-α) promoter polymorphism in relation to infection with the cytotoxin associated gene A (cagA) subtype of Helicobacter pylori within a dyspeptic Korean population. Methods—Eighty three patients with gastric disease and 113 healthy control...

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Bibliographic Details
Published in:Journal of clinical pathology 2001-09, Vol.54 (9), p.703-706
Main Authors: Yea, S S, Yang, Y-I, Jang, W H, Lee, Y J, Bae, H-S, Paik, K-H
Format: Article
Language:English
Subjects:
Antigens, Bacterial
Bacterial Proteins - genetics
Bacterial Typing Techniques
Biological and medical sciences
Biopsy
cagA
Confidence intervals
Gastric cancer
Gastroenterology. Liver. Pancreas. Abdomen
Genetic polymorphisms
Genetic Predisposition to Disease
Genotype
Genotype & phenotype
Helicobacter infections
Helicobacter Infections - complications
Helicobacter Infections - genetics
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - classification
Helicobacter pylori - genetics
Humans
Infections
Medical sciences
Other diseases. Semiology
Patients
Physiological aspects
Polymerase Chain Reaction
Polymorphism
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Promoter Regions, Genetic
Statistical analysis
Stomach Diseases - genetics
Stomach Diseases - microbiology
Stomach Neoplasms - genetics
Stomach Neoplasms - microbiology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tropical medicine
Tumor necrosis factor
Tumor Necrosis Factor-alpha - genetics
Tumor necrosis factor-TNF
tumour necrosis factor α
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Summary:Aims—To assess the importance of tumour necrosis factor α (TNF-α) promoter polymorphism in relation to infection with the cytotoxin associated gene A (cagA) subtype of Helicobacter pylori within a dyspeptic Korean population. Methods—Eighty three patients with gastric disease and 113 healthy controls were studied. The DNA from gastric biopsy specimens was analysed by H pylori specific and cagA specific polymerase chain reaction (PCR). To characterise TNF-α polymorphism at positions −308 and −238, PCR based restriction fragment length polymorphism analysis was performed. Results—Helicobacter pylori infection was closely correlated with G to A transition at position −308 of the TNF-α promoter when compared with healthy controls (odds ratio (OR), 2.912; 95% confidence interval (CI), 1.082 to 7.836; p = 0.034). Although TNF-α −308 polymorphism in patients with H pylori was not significantly different from that in patients without H pylori, the −308A polymorphism was strongly associated with H pylori cagA subtype infection when compared with the polymorphism in cagA negative H pylori infection (OR, 8.757; 95% CI, 1.413 to 54.262; p = 0.019) and healthy controls (OR, 3.683; 95% CI, 1.343 to 10.101; p = 0.011). G to A genetic change at position −238 of the TNF-α gene was not significantly associated with H pylori cagA subtype infection. In addition, genetic polymorphisms at both sites of the TNF-α promoter in patients with H pylori infection did not correlate with the severity of disease. Conclusion—TNF-α −308A polymorphism was significantly related to infection with the H pylori cagA subtype in Korean patients with gastric disease.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.54.9.703