Expression of ADAMs and their inhibitors in sputum from patients with asthma

ADAMs (a disintegrin and metalloprotease) constitute a family of cell surface proteins containing disintegrin and metalloprotease domains which associate features of adhesion molecules and proteases. ADAMTSs (a disintegrin and metalloprotease with thrombospondin motifs) bear thrombospondin type I mo...

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Bibliographic Details
Published in:Molecular medicine (Cambridge, Mass.) Mass.), 2006-07, Vol.12 (7-8), p.171-179
Main Authors: Paulissen, Geneviève, Rocks, Natacha, Quesada-Calvo, Florence, Gosset, Philippe, Foidart, Jean-Michel, Noel, Agnès, Louis, Renaud, Cataldo, Didier D
Format: Article
Language:English
Subjects:
ADAM Proteins - antagonists & inhibitors
ADAM Proteins - genetics
ADAM Proteins - metabolism
Adult
Aged
Asthma - genetics
Asthma - metabolism
Biochemistry, biophysics & molecular biology
Biochimie, biophysique & biologie moléculaire
Blotting, Western
Case-Control Studies
Cell Count
Female
Gene Expression Regulation
GPI-Linked Proteins
Human health sciences
Humans
Immunohistochemistry
Inflammation
Laboratory medicine & medical technology
Life sciences
Lipopolysaccharides - metabolism
Male
Membrane Glycoproteins - metabolism
Middle Aged
Médecine de laboratoire & technologie médicale
Receptors, IgE - immunology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sciences de la santé humaine
Sciences du vivant
Sputum - cytology
Sputum - metabolism
Subcellular Fractions
Tissue Inhibitor of Metalloproteinases - genetics
Tissue Inhibitor of Metalloproteinases - metabolism
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Summary:ADAMs (a disintegrin and metalloprotease) constitute a family of cell surface proteins containing disintegrin and metalloprotease domains which associate features of adhesion molecules and proteases. ADAMTSs (a disintegrin and metalloprotease with thrombospondin motifs) bear thrombospondin type I motifs in C-terminal extremity, and most of them are secreted proteins. Because genetic studies have shown that ADAM-33 gene polymorphisms are associated with asthma, we designed this study to assess mRNA expression profile of several ADAM and ADAMTS proteases in sputum from patients with asthma and to investigate the relationship between expression of these proteases and asthma-associated inflammation and airway obstruction. mRNA expression profile of selected ADAM and ADAMTS proteinases (ADAM-8, -9, -10, -12, -15, -17, and -33; ADAMTS-1, -2, -15, -16, -17, -18, and -19), their physiological inhibitors TIMP-1 and TIMP-3, and RECK, a membrane-anchored MMP activity regulator, was obtained by RT-PCR analysis performed on cells collected by sputum induction from 21 patients with mild to moderate asthma and 17 healthy individuals. mRNA levels of ADAM-8, ADAM-9, ADAM-12, TIMP-1, and TIMP-3 were significantly increased, whereas mRNA levels coding for ADAMTS-1, ADAMTS-15, and RECK were significantly decreased in patients with asthma compared with control patients. ADAM-8 expression was negatively correlated with the forced expiratory volume at the first second (FEV(1)) (r = -0.57, P < 0.01), whereas ADAMTS-1 and RECK expressions were positively correlated to FEV(1) (r = 0.45, P < 0.05, and r = 0.55, P = 0.01, respectively). We conclude that expression of ADAMs and ADAMTSs and their inhibitors is modulated in airways from patients with asthma and that these molecules may play a role in the pathogenesis of asthma.
ISSN:1076-1551
1528-3658
1528-3658
DOI:10.2119/2006-00028.Paulissen