Isolated plant nuclei nucleate microtubule assembly: the nuclear surface in higher plants has centrosome-like activity

In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain hig...

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Bibliographic Details
Published in:The Plant cell 1994-08, Vol.6 (8), p.1099-1106
Main Authors: Stoppin, V. (Universite Louis Pasteur, Strasbourg, France), Vantard, M, Schmit, A.C, Lambert, A.M
Format: Article
Language:English
Subjects:
Cell nucleus
Centrosomes
Corn
ESTRUCTURA CELULAR
GLICOPROTEINAS
GLYCOPROTEINE
IMMUNOLOGIE
INMUNOLOGIA
Interphase
Microscopy
Microtubules
NOYAU CELLULAIRE
Nuclear membrane
Nucleation
NUCLEO
ORGANITE CELLULAIRE
ORGANULOS CITOPLASMICOS
Plant cells
Plants
STRUCTURE CELLULAIRE
ZEA MAYS
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Summary:In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain highly debatable. A large body of evidence indicates that plant MTs emerge from the nuclear periphery. In this study, we developed an in vitro assay in which isolated maize nuclei nucleate MT assembly at a tubulin concentration (14 micromolar of neurotubulin) that is not efficient for spontaneous MT assembly. No MT-stabilizing agents, such as taxol or dimethyl sulfoxide, were used. Our model provides evidence that the nuclear surface functions as a MT-nucleating site in higher plant cells A monoclonal antibody raised against a pericentriolar antigen immunostained the surface of isolated nuclei, and a 100-kD polypeptide in 4 M urea-treated nuclear extracts was detected
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.6.8.1099