Water deficit rapidly stimulates the activity of a protein kinase in the elongation zone of the maize primary root

The mechanisms by which plants detect water deficit and transduce that signal into adaptive responses is unknown. In maize (Zea mays L.) seedlings, primary roots adapt to low water potentials such that substantial rates of elongation continue when shoot growth is completely inhibited. In this study,...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1997-01, Vol.113 (1), p.219-226
Main Authors: Conley, T.R. (Oklahoma City University, Oklahoma City, OK.), Sharp, R.E, Walker, J.C
Format: Article
Language:English
Subjects:
ABA
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ADAPTACION
ADAPTATION
Biological and medical sciences
Cell Biology and Signal Transduction
CELLULE
CELULAS
CONTENIDO PROTEICO
Corn
CRECIMIENTO
CROISSANCE
Dehydration
ESTIMULO
ESTRES DE SEQUIA
FOSFOPROTEINAS
Fundamental and applied biological sciences. Psychology
Gels
Gene expression regulation
PHOSPHOPROTEINE
Phosphorylation
Plant physiology and development
Plant roots
Plants
PLANTULAS
PLANTULE
PROTEINA QUINASA
PROTEINE KINASE
RACINE
RAICES
RELACIONES PLANTA AGUA
RELATION PLANTE EAU
Root growth
Seedlings
SINTESIS DE PROTEINAS
STIMULUS
STRESS DU A LA SECHERESSE
SYNTHESE PROTEIQUE
TENEUR EN PROTEINES
Vermiculite
Water and solutes. Absorption, translocation and permeability
ZEA MAYS
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mechanisms by which plants detect water deficit and transduce that signal into adaptive responses is unknown. In maize (Zea mays L.) seedlings, primary roots adapt to low water potentials such that substantial rates of elongation continue when shoot growth is completely inhibited. In this study, in-gel protein kinase assays were used to determine whether protein kineses in the elongation zone of the primary root undergo activation or inactivation in response to water deficit. Multiple differences were detected in the phosphoprotein content of root tips of water-stressed compared with well-watered seedlings. Protein kinase assays identified water-deficit-activated protein kinases, including a 45-kD, Ca2+-independent serine/threonine protein kinase. Water-deficit activation of this kinase occurred within 30 min after transplanting seedlings to conditions of low water potential and was localized to the elongation zone, was independent of ABA accumulation, and was unaffected by cycloheximide-mediated inhibition of protein translation. These results provide evidence that the 45-kD protein kinase acts at an early step in the response of maize primary roots to water deficit and is possibly involved in regulating the adaptation of root growth to low water potential
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.113.1.219