Frequencies, timing, and spatial patterns of co-suppression of nitrate reductase and nitrite reductase in transgenic tobacco plants

Frequencies, timing, and spatial patterns of co-suppression of the nitrate (Nia) and nitrite (Nii) genes were analyzed in transgenic tobacco (Nicotiana tabacum) plants carrying either Nia or Ni; cDNAs under the control of the 35S promoter, or a Nii gene with its own regulatory signals (promoter, int...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1996-12, Vol.112 (4), p.1447-1456
Main Authors: Palauqui, J.C. (Institut National de la Recherche Agronomique, Versailles, France.), Elmayan, T, Dorlhac de Borne, F, Crete, P, Charles, C, Vaucheret, H
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Biotechnology
CHLOROSE
CLOROSIS
Complementary DNA
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
FENOLOGIA
FEUILLE
Fundamental and applied biological sciences. Psychology
GENE
Gene Regulation and Molecular Genetics
GENES
Genetic engineering
Genetic loci
Genetic technics
Genetics
HOJAS
Hypochromic anemia
INTERACCION DE GENES
INTERACTION GENIQUE
Leaves
Life Sciences
Messenger RNA
Methods. Procedures. Technologies
NICOTIANA TABACUM
NITRATE REDUCTASE
Nitrates
NITRATO REDUCTASA
NITRITE REDUCTASE
NITRITO REDUCTASA
PHENOLOGIE
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
Plants genetics
STADE DE DEVELOPPEMENT VEGETAL
TRANSCRIPCION
TRANSCRIPTION
Transgenes
Transgenic animals and transgenic plants
Transgenic plants
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Frequencies, timing, and spatial patterns of co-suppression of the nitrate (Nia) and nitrite (Nii) genes were analyzed in transgenic tobacco (Nicotiana tabacum) plants carrying either Nia or Ni; cDNAs under the control of the 35S promoter, or a Nii gene with its own regulatory signals (promoter, introns, and terminator) cloned downstream of two copies of the enhancer of the 35S promoter. We show that (a) the frequencies of transgenic lines affected by cosuppression are similar for the three constructs, ranging from 19 to 25%; (b) Nia and Nii co-suppression are triggered stochastically during a phenocritical period of 2 weeks between germination and flowering; (c) the timing of co-suppression (i.e. the percentage of isogenic plants affected by co-suppression reported as a function of the number of days of culture) differs from one transgenic line to another; (d) the percentage of isogenic plants affected by cosuppression is increased by growing the plants in vitro prior to their transfer to the greenhouse and to the field; and (e) at the end of the culture period, plants are either unaffected, completely co-suppressed, or variegated. Suppressed and nonsuppressed parts of these variegated plants are separated by a vertical plane through the stem in Nia co-suppression, and separated by a horizontal plane in Nii co-suppression
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.112.4.1447