Adenylosuccinate synthetase: site of action of hydantocidin, a microbial phytotoxin

The site of action of hydantocidin was probed using Arabidopsis thaliana plants growing on agar plates. Herbicidal effects were reversed when the agar medium was supplemented with AMP, but not IMP or GMP, suggesting that hydantocidin blocked the two-step conversion of IMP to AMP in the de novo purin...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1996-03, Vol.110 (3), p.753-758
Main Authors: Siehl, D.L. (Sandoz Agro, Inc., Palo Alto, CA.), Subramanian, M.V, Walters, E.W, Lee, S.F, Anderson, R.J, Toschi, A.G
Format: Article
Language:English
Subjects:
ACIDE SUCCINIQUE
ACIDO SUCCINICO
ACTIVIDAD CATALITICA
ACTIVITE CATALYTIQUE
ADENOSINE MONOPHOSPHATE
Adenosine Monophosphate - biosynthesis
ADENOSINMONOFOSFATO
Adenylosuccinate Lyase - drug effects
Adenylosuccinate Synthase - drug effects
Antidotes
Arabidopsis - drug effects
Arabidopsis - enzymology
Arabidopsis - growth & development
ARABIDOPSIS THALIANA
Biological and medical sciences
Biosynthesis
Chemical control
Corn
CULTIVO DE CELULAS
CULTURE DE CELLULE
DESHERBAGE
ENZIMAS
ENZYME
Enzyme Inhibitors - pharmacology
Enzymes
ESCARDA
ESTRUCTURA QUIMICA
FITOTOXINAS
Fundamental and applied biological sciences. Psychology
Glycine - analogs & derivatives
Glycine - pharmacology
HERBICIDAS
HERBICIDE
Herbicides
Herbicides - chemistry
Herbicides - pharmacology
Hydantoins - chemistry
Hydantoins - pharmacology
INHIBIDORES DEL CRECIMIENTO
INHIBITEUR DE CROISSANCE
Inosine Monophosphate - metabolism
MEDIO DE CULTIVO
MILIEU DE CULTURE
Mode of action
NUCLEOTIDE
NUCLEOTIDOS
Parasitic plants. Weeds
Pentosephosphates - pharmacology
Phytopathology. Animal pests. Plant and forest protection
PHYTOTOXINE
Plant growth
Plants
Purines
Rapid Communication
REACCIONES QUIMICAS
REACTION CHIMIQUE
Seedlings
STREPTOMYCES
STRUCTURE CHIMIQUE
VIA BIOQUIMICA DEL METABOLISMO
VOIE BIOCHIMIQUE DU METABOLISME
Weeds
Zea mays - enzymology
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Summary:The site of action of hydantocidin was probed using Arabidopsis thaliana plants growing on agar plates. Herbicidal effects were reversed when the agar medium was supplemented with AMP, but not IMP or GMP, suggesting that hydantocidin blocked the two-step conversion of IMP to AMP in the de novo purine biosynthesis pathway. Hydantocidin itself did not inhibit adenylosuccinate synthetase or adenylosuccinate lyase isolated from Zea mays. However, a phosphorylated derivative of hydantocidin, N-acetyl-5'-phosphohydantocidin, was a potent inhibitor of the synthetase but not of the lyase. These results identify the site of action of hydantocidin and establish adenylosuccinate synthetase as an herbicide target of commercial potential
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.110.3.753