An efficient genetic screen in Drosophila to identify nuclear-encoded genes with mitochondrial function

We conducted a screen for glossy-eye flies that fail to incorporate BrdU in the third larval instar eye disc but exhibit normal neuronal differentiation and isolated 23 complementation groups of mutants. These same phenotypes were previously seen in mutants for cytochrome c oxidase subunit Va. We ha...

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Published in:Genetics (Austin) 2006-09, Vol.174 (1), p.525-533
Main Authors: Liao, T S Vivian, Call, Gerald B, Guptan, Preeta, Cespedes, Albert, Marshall, Jamie, Yackle, Kevin, Owusu-Ansah, Edward, Mandal, Sudip, Fang, Q Angela, Goodstein, Gelsey L, Kim, William, Banerjee, Utpal
Format: Article
Language:English
Subjects:
Alkyl and Aryl Transferases - genetics
Animals
Apoptosis
Arginine-tRNA Ligase - genetics
Cell cycle
Cell Nucleus - genetics
Chromosome Mapping - methods
Crosses, Genetic
Drosophila
Drosophila - genetics
Embryo, Nonmammalian
Eye - embryology
Eye - growth & development
Female
Genetic Testing - methods
Genotype & phenotype
Insect Proteins - genetics
Lyases - genetics
Male
Mitochondria - metabolism
Mitochondrial DNA
Mitochondrial Proteins - biosynthesis
Mitochondrial Proteins - genetics
Models, Biological
Mutation
Nitrogenous Group Transferases - genetics
Proteins
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Summary:We conducted a screen for glossy-eye flies that fail to incorporate BrdU in the third larval instar eye disc but exhibit normal neuronal differentiation and isolated 23 complementation groups of mutants. These same phenotypes were previously seen in mutants for cytochrome c oxidase subunit Va. We have molecularly characterized six complementation groups and, surprisingly, each encodes a mitochondrial protein. Therefore, we believe our screen to be an efficient method for identifying genes with mitochondrial function.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.106.061705