Activation Tagging in Arabidopsis

Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have bee...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2000-04, Vol.122 (4), p.1003-1013
Main Authors: Weigel, Detlef, Ahn, Ji Hoon, Blázquez, Miguel A., Borevitz, Justin O., Christensen, Sioux K., Fankhauser, Christian, Ferrándiz, Cristina, Kardailsky, Igor, Malancharuvil, Elizabeth J., Neff, Michael M., Jasmine Thuy Nguyen, Sato, Shusei, Wang, Zhi-Yong, Xia, Yiji, Dixon, Richard A., Harrison, Maria J., Lamb, Chris J., Yanofsky, Martin F., Chory, Joanne
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - virology
Base Sequence
Biological and medical sciences
Biotechnology
Breakthrough Technologies
Cauliflower mosaic virus
Caulimovirus - genetics
DNA
DNA Primers
DNA, Bacterial
Enhancer Elements, Genetic
Flowering
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genetic engineering
Genetic loci
Genetic mutation
Genetic technics
Genetic Vectors
Genomics
glufosinate
kanamycin
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular genetics
Phenotype
Phenotypes
Plant cells
Plants
Plasmids
Promoter Regions, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant mutants with various phenotypes have been isolated. Analysis of a subset of mutants has shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to 3.6 kb. In at least one case, the CaMV 35S enhancers led primarily to an enhancement of the endogenous expression pattern rather than to constitutive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the complete CaMV 35S promoter. This has important implications for the spectrum of genes that will be discovered by this method.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.122.4.1003