Differential expression of the calcium‐binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study

SUMMARY MRP14 and MRP8 are well‐characterized calcium‐binding proteins present in myeloid cells and mononuclear phagocytes. These antigens can easily be visualized in paraffin‐embedded tissue, making use of monospecific polyclonal antibodies. This study evaluates MRP14 and MRP8 expression in mononuc...

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Published in:Clinical and experimental immunology 1990-07, Vol.81 (1), p.123-126
Main Authors: DELABIE, J., WOLF‐PEETERS, C. DE, OORD, J. J. VAN DEN, DESMET, V. J.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Biological and medical sciences
Calcium-Binding Proteins - metabolism
Calgranulin A
Calgranulin B
delayed hypersensitivity
Epithelium - metabolism
Foreign-Body Reaction - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
granuloma
Granuloma - metabolism
Histiocytes - metabolism
Humans
Immunobiology
Immunoenzyme Techniques
immunohistochemistry
Lymph Nodes - metabolism
Macromolecular Substances
Organs and cells involved in the immune response
Phagocytes - immunology
Skin - metabolism
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Summary:SUMMARY MRP14 and MRP8 are well‐characterized calcium‐binding proteins present in myeloid cells and mononuclear phagocytes. These antigens can easily be visualized in paraffin‐embedded tissue, making use of monospecific polyclonal antibodies. This study evaluates MRP14 and MRP8 expression in mononuclear phagocytes in various granulomatous conditions. MRP14 is strongly expressed in all granulomatous conditions. MRP8 is variably expressed. Mononuclear phagocytes in granulomas of foreign body type, cat‐scratch discase and erythema nodosum strongly express MRP8. In contrast, MRP8 expression is weak or absent in mononuclear phagocytes of sarcoidosis and tuberculosis. These results show differences in immunophenotype between non‐phagocytic mononuclear phagocytes in delayed hypersensitivity type granulomas and phagocytic mononuclear phagocytes in non‐hypersensitivity and non‐immunological granulomas.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1990.tb05301.x