Parathyroid hormone-related protein in tissues of the emerging frog (Rana temporaria): immunohistochemistry and in situ hybridisation

Using antiserum to human parathyroid hormone-related protein (1–16) [PTHrP(1–16)] we have examined tissues of the common frog (Rana temporaria) for the presence of immunoreactive PTHrP (irPTHrP) at the stage of emergence from water to land. irPTHrP was detected in dorsal and ventral stratum granulos...

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Bibliographic Details
Published in:American journal of anatomy 1997-02, Vol.190 (2), p.229-238
Main Authors: DANKS, J. A., McHALE, J. C., MARTIN, T. J., INGLETON, P. M.
Format: Article
Language:English
Subjects:
amphibia
Animals
development
Female
Gene Expression
Hypothalamus - chemistry
Immunohistochemistry
In Situ Hybridization
Muscle, Skeletal - chemistry
Olfactory Bulb - chemistry
Ovary - chemistry
Parathyroid Hormone - analysis
Parathyroid Hormone - genetics
Parathyroid Hormone-Related Protein
Pituitary Gland - chemistry
Proteins - analysis
Proteins - genetics
PTHrP
Rana temporaria - growth & development
Rana temporaria - metabolism
RNA, Messenger - analysis
Skin - chemistry
Tissue Distribution
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Summary:Using antiserum to human parathyroid hormone-related protein (1–16) [PTHrP(1–16)] we have examined tissues of the common frog (Rana temporaria) for the presence of immunoreactive PTHrP (irPTHrP) at the stage of emergence from water to land. irPTHrP was detected in dorsal and ventral stratum granulosum of the skin, in the developing ovary, striated muscle and the choroid plexus epithelium of the brain as well as in the olfactory gland epithelium and olfactory lobe neurons of the brain. In the pituitary and hypothalamus irPTHrP protein could be demonstrated in the median eminence, infundibular stem and principally in the neural lobe and pars distalis of the pituitary with weak reaction in the pars intermedia. In situ hybridisation of the same tissues with an oligonucleotide probe to chicken PTHrP 55–65 clearly showed the presence of mRNA for PTHrP-like molecule in all the tissues containing irPTHrP. There was a major inconsistency in the pituitary in that the highest level of gene expression, assessed by in situ hybridisation, was found in the pars intermedia with only very low expression in the pars distalis and neural lobe and undetectable levels in the infundibular stem and median eminence. These observations suggest that tissues of the frog synthesise a PTHrP-like molecule but that in the pituitary the pars intermedia cells may export the protein to cells in other regions of the pituitary and hypothalamus.
ISSN:0002-9106
0021-8782
1553-0795
1469-7580
DOI:10.1046/j.1469-7580.1997.19020229.x