Suppressor analysis of mutations in the 5'-untranslated region of COB mRNA identifies components of general pathways for mitochondrial mRNA processing and decay in Saccharomyces cerevisiae

The cytochrome b gene in Saccharomyces cerevisiae, COB, is encoded by the mitochondrial genome. Nuclear-encoded Cbp1 protein is required specifically for COB mRNA stabilization. Cbp1 interacts with a CCG element in a 64-nucleotide sequence in the 5'-untranslated region of COB mRNA. Mutation of...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 1999-04, Vol.151 (4), p.1315-1325
Main Authors: Chen, W. (University of Arizona, Tucson, AZ.), Islas-Osuna, M.A, Dieckmann, C.L
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Alleles
Apoproteins - genetics
ARN MENSAJERO
ARN MESSAGER
CITOCROMO B
CYTOCHROME B
Cytochrome b Group - genetics
Cytochromes b
DOMINANCE
GENE
Gene Expression
GENE LOCATION
GENES
Genes, Fungal
Genetic Linkage
GENETICA MITOCONDRIAL
Genetics
GENETIQUE MITOCHONDRIALE
INHIBITOR GENES
LINKAGE GROUPS
LOCALISATION DE GENE
LOCALIZACION DE GENES
MESSENGER RNA
MITOCHONDRIAL GENETICS
MUTACION
MUTATION
Phenotype
Proteins
Ribonucleic acid
RNA
RNA Processing, Post-Transcriptional
RNA, Fungal - genetics
RNA, Fungal - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Suppression, Genetic
SUPPRESSOR MUTATIONS
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cytochrome b gene in Saccharomyces cerevisiae, COB, is encoded by the mitochondrial genome. Nuclear-encoded Cbp1 protein is required specifically for COB mRNA stabilization. Cbp1 interacts with a CCG element in a 64-nucleotide sequence in the 5'-untranslated region of COB mRNA. Mutation of any nucleotide in the CCG causes the same phenotype as cbp1 mutations, i.e., destabilization of both COB precursor and mature message. In this study, eleven nuclear suppressors of single-nucleotide mutations in CCG were isolated and characterized. One dominant suppressor is in CBP1, while the other 10 semidominant suppressors define five distinct linkage groups. One group of four mutations is in PET127, which is required for 5' end processing of several mitochondrial mRNAs. Another mutation is linked to DSS1, which is a subunit of mitochondrial 3' --> 5' exoribonuclease. A mutation linked to the SOC1 gene, previously defined by recessive mutations that suppress cbp1 ts alleles and stabilize many mitochondrial mRNAs, was also isolated. We hypothesize that the products of the two uncharacterized genes also affect mitochondrial RNA turnover.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/151.4.1315