Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p Homolog, Is an Essential ATPase in RSC and Differs From Snf/Swi in Its Interactions With Histones and Chromatin-Associated Proteins

The essential Sth1p is the protein most closely related to the conserved Snf2p/Swi2p in Saccharomyces cerevisiae. Sth1p purified from yeast has a DNA-stimulated ATPase activity required for its function in vivo. The finding that Sth1p is a component of a multiprotein complex capable of ATP-dependent...

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Bibliographic Details
Published in:Genetics (Austin) 1998-11, Vol.150 (3), p.987-1005
Main Authors: Du, Jian, Nasir, Irem, Benton, Benjamin K, Kladde, Michael P, Laurent, Brehon C
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Amino Acid Sequence
Cell Cycle - genetics
Cell Cycle Proteins
Chromatin - genetics
Chromatin - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Enzymes
Escherichia coli - genetics
Fungal Proteins - genetics
Fungal Proteins - metabolism
Genetics
Histones - genetics
Histones - metabolism
Molecular Sequence Data
Mutation
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Transcription Factors - genetics
Transcription Factors - metabolism
Yeast
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Summary:The essential Sth1p is the protein most closely related to the conserved Snf2p/Swi2p in Saccharomyces cerevisiae. Sth1p purified from yeast has a DNA-stimulated ATPase activity required for its function in vivo. The finding that Sth1p is a component of a multiprotein complex capable of ATP-dependent remodeling of the structure of chromatin (RSC) in vitro, suggests that it provides RSC with ATP hydrolysis activity. Three sth1 temperature-sensitive mutations map to the highly conserved ATPase/helicase domain and have cell cycle and non-cell cycle phenotypes, suggesting multiple essential roles for Sth1p. The Sth1p bromodomain is required for wild-type function; deletion mutants lacking portions of this region are thermosensitive and arrest with highly elongated buds and 2C DNA content, indicating perturbation of a unique function. The pleiotropic growth defects of sth1-ts mutants imply a requirement for Sth1p in a general cellular process that affects several metabolic pathways. Significantly, an sth1-ts allele is synthetically sick or lethal with previously identified mutations in histones and chromatin assembly genes that suppress snf/swi, suggesting that RSC interacts differently with chromatin than Snf/Swi. These results provide a framework for understanding the ATP-dependent RSC function in modeling chromatin and its connection to the cell cycle.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/150.3.987