Abortive transposition by a group II intron in yeast mitochondria

Group II intron homing in yeast mitochondria is initiated at active target sites by activities of intron-encoded ribonucleoprotein (RNP) particles, but is completed by competing recombination and repair mechanisms. Intron aI1 transposes in haploid cells at low frequency to target sites in mtDNA that...

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Bibliographic Details
Published in:Genetics (Austin) 2004-09, Vol.168 (1), p.77-87
Main Authors: Dickson, L, Connell, S, Huang, H.R, Henke, R.M, Liu, L, Perlman, P.S
Format: Article
Language:English
Subjects:
Crosses, Genetic
Cyclooxygenase 1
DNA Transposable Elements - genetics
DNA, Complementary - genetics
Gene Rearrangement - genetics
Genetics
Introns - genetics
Investigations
Mitochondria - genetics
Mitochondrial DNA
Models, Genetic
Movement
Polymorphism, Restriction Fragment Length
Prostaglandin-Endoperoxide Synthases - genetics
Ribonucleoproteins - genetics
Ribonucleoproteins - metabolism
Yeast
Yeasts - genetics
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Summary:Group II intron homing in yeast mitochondria is initiated at active target sites by activities of intron-encoded ribonucleoprotein (RNP) particles, but is completed by competing recombination and repair mechanisms. Intron aI1 transposes in haploid cells at low frequency to target sites in mtDNA that resemble the exon 1-exon 2 (E1/E2) homing site. This study investigates a system in which aI1 can transpose in crosses (i.e., in trans). Surprisingly, replacing an inefficient transposition site with an active E1/E2 site supports
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.104.027003