Evaluation of porcine ileum models of enterocyte infection by Lawsonia intracellularis

The early interaction of Lawsonia intracellularis with host cells was examined with the use of porcine ileum models. Two conventional swine were anesthetized, and ligated ileum loops were prepared during abdominal surgery. The loops were inoculated with 10⁸ L. intracellularis or saline. After 60 min...

Full description

Saved in:
Bibliographic Details
Published in:Canadian journal of veterinary research 2006-04, Vol.70 (2), p.155-159
Main Authors: McOrist, S, Gebhart, C.J, Bosworth, B.T
Format: Article
Language:English
Subjects:
Animals
Cell Division
cell proliferation
Desulfovibrionaceae Infections - microbiology
Desulfovibrionaceae Infections - pathology
Desulfovibrionaceae Infections - veterinary
Disease Models, Animal
enterocytes
Enterocytes - microbiology
Enterocytes - physiology
Erythrocytes - microbiology
ileum
Ileum - cytology
Ileum - microbiology
Ileum - pathology
Ileum - ultrastructure
in vivo studies
intestinal crypts
Lawsonia Bacteria - pathogenicity
Lawsonia intracellularis
Mice
Microscopy, Electron, Transmission - veterinary
porcine proliferative enteritis
Short Communications
Swine
Swine Diseases
Transplantation, Heterologous - veterinary
xenotransplantation
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The early interaction of Lawsonia intracellularis with host cells was examined with the use of porcine ileum models. Two conventional swine were anesthetized, and ligated ileum loops were prepared during abdominal surgery. The loops were inoculated with 10⁸ L. intracellularis or saline. After 60 min, samples of each loop were processed for routine histologic and electron microscopic study. Histologic and ultrathin sections of all the loops appeared normal, with no apposition of bacteria and host cells or bacterial entry events in any loop. Portions of ileum from a single gnotobiotic piglet were introduced as xenografts into the subcutis of each flank of 5 weaned mice with severe combined immunodeficiency disease. After 4 wk, 10⁸ L. intracellularis were inoculated into each of 4 viable xenografts with a sterile needle; the other 3 viable xenografts received saline. Histologic and ultrathin sections of all the xenografts 3 wk after inoculation showed relatively normal porcine intestinal architecture, with normal crypts, crypt cell differentiation, and low villous structures; the xenografts treated with the bacteria also showed intracytoplasmic L. intracellularis within crypt and villous epithelial cells. Thus, entry of L. intracellularis into target epithelial cells and multiplication may not be sufficient alone to directly cause cell proliferation. A proliferative response may require active division of crypt cells and differentiation in conjunction with L. intracellularis growth.
ISSN:0830-9000