A Lipid Transfer-Like Protein Is Necessary for Lily Pollen Tube Adhesion to an in vitro Stylar Matrix

Flowering plants possess specialized extracellular matrices in the female organs of the flower that support pollen tube growth and sperm cell transfer along the transmitting tract of the gynoecium. Transport of the pollen tube cell and the sperm cells involves a cell adhesion and migration event in...

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Bibliographic Details
Published in:The Plant cell 2000-01, Vol.12 (1), p.151-163
Main Authors: Park, Sang-Youl, Jauh, Guang-Yuh, Mollet, Jean-Claude, Eckard, Kathleen J., Nothnagel, Eugene A., Walling, Linda L., Lord, Elizabeth M.
Format: Article
Language:English
Subjects:
Adhesion
Amino Acid Sequence
Base Sequence
Biochemistry
Biochemistry, Molecular Biology
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Adhesion
Cell Behavior
Cellular Biology
Chemical Sciences
DNA Primers - genetics
Epidermal cells
Extracellular Matrix - metabolism
Gels
Life Sciences
Liliaceae - anatomy & histology
Liliaceae - genetics
Liliaceae - metabolism
Lipid Metabolism
Lipids
Microscopy, Electron
Models, Biological
Molecular biology
Molecular Sequence Data
Molecules
Plant cells
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Pollen tubes
Polymers
Proteins
Reproductive Biology
Sequence Homology, Amino Acid
Sexual reproduction
Styles
Vegetal Biology
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Summary:Flowering plants possess specialized extracellular matrices in the female organs of the flower that support pollen tube growth and sperm cell transfer along the transmitting tract of the gynoecium. Transport of the pollen tube cell and the sperm cells involves a cell adhesion and migration event in species such as lily that possess a transmitting tract epidermis in the stigma, style, and ovary. A bioassay for adhesion was used to isolate from the lily stigma/stylar exudate the components that are responsible for in vivo pollen tube adhesion. At least two stylar components are necessary for adhesion: a large molecule and a small (9 kD) protein. In combination, the two molecules induced adhesion of pollen tubes to an artificial stylar matrix in vitro. The 9-kD protein was purified, and its corresponding cDNA was cloned. This molecule shares some similarity with plant lipid transfer proteins. Immunolocalization data support its role in facilitating adhesion of pollen tubes to the stylar transmitting tract epidermis.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.12.1.151