Interactions of dendritic cells with fibronectin and endothelial cells

We studied the phenotypic characteristics of spontaneously migrated skin dendritic cells (sDC) and monocyte‐derived dendritic cells (moDC), generated under different culture conditions, and their interactions with fibronectin (FN) and endothelial cells. Monocyte‐derived dendritic cells were obtained...

Full description

Saved in:
Bibliographic Details
Published in:Immunology 1998-10, Vol.95 (2), p.283-290
Main Authors: Jancic, C, Chuluyan, H E, Morelli, A, Larregina, A, Kolkowski, E, Saracco, M, Barboza, M, Leiva, W S, Fainboim, L
Format: Article
Language:English
Subjects:
Animals
Cattle
Cell Adhesion - physiology
Cell Communication - physiology
Cell Movement - physiology
Cells, Cultured
Culture Media
Dendritic Cells - metabolism
Dendritic Cells - physiology
Endothelium - physiology
Fibronectins - physiology
Flow Cytometry
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Humans
Integrins - metabolism
Interleukin-4 - pharmacology
Monocytes - immunology
Skin - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the phenotypic characteristics of spontaneously migrated skin dendritic cells (sDC) and monocyte‐derived dendritic cells (moDC), generated under different culture conditions, and their interactions with fibronectin (FN) and endothelial cells. Monocyte‐derived dendritic cells were obtained after culturing monocytes with granulocyte–macrophage colony‐stimulating factor (GM‐CSF) (800 U/ml) and interleukin‐4 (IL‐4) (500 U/ml) with either 10% fetal bovine serum (FBS) or 10% allogeneic human serum (HS). Regardless of the type of serum used, the majority of moDC expressed human leucocyte antigen‐DR (HLA‐DR) and CD86. On day 5 of incubation, 20–67% of moDC cultured in the presence of HS (HS‐moDC) expressed CD1a, b and c versus 94–97% when cultured in the presence of FBS (FBS‐moDC). DC showed a differential gradient of adhesion to FN: FBS‐moDC>HS‐moDC>sDC≈monocytes. Both FBS‐moDC and HS‐moDC were strongly positive for CD49e (α5‐integrin) and CD29 (β1‐integrin) but negative for CD49d (α4‐integrin). A monoclonal antibody (mAb) against CD49e blocked the adhesion of both types of moDC to FN. Although both FBS‐moDC and HS‐moDC attached to endothelium (a 76% and 63% increase, respectively), only HS‐moDC were able to migrate through non‐activated endothelium. Overall, these results suggest that spontaneously migrated sDC are less adherent to FN than moDC, that HS and FBS induce differences in CD1 expression, that HS‐moDC are less adhesive to FN and endothelial cells but more motile than FBS‐moDC, and that α5β1‐integrin is the molecule involved in moDC adhesion to FN.
ISSN:0019-2805
1365-2567
DOI:10.1046/j.1365-2567.1998.00586.x