Macrophage migration inhibitory factor induces phagocytosis of foreign particles by macrophages in autocrine and paracrine fashion

Exposure to foreign particles sometimes causes inflammatory reactions through production of cytokines and chemoattractants by phagocytic cells. In this study, we focused on macrophage migration inhibitory factor (MIF) to evaluate its pathophysiological role in the phagocytic process. Immunohistochem...

Full description

Saved in:
Bibliographic Details
Published in:Immunology 1997-09, Vol.92 (1), p.131-137
Main Authors: ONODERA, S., SUZUKI, K., MATSUNO, T., KANEDA, K., TAKAGI, M., NISHIHIRA, J.
Format: Article
Language:English
Subjects:
Animals
Autocrine Communication - immunology
Blotting, Northern
Cell Communication - immunology
Gene Expression
Hip Prosthesis
Humans
Immunoenzyme Techniques
Macrophage Activation - immunology
Macrophage Migration-Inhibitory Factors - genetics
Macrophage Migration-Inhibitory Factors - immunology
Macrophages - immunology
Mice
Paracrine Communication - immunology
Phagocytosis - immunology
Prosthesis Failure
RNA, Messenger - genetics
Synovial Membrane - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exposure to foreign particles sometimes causes inflammatory reactions through production of cytokines and chemoattractants by phagocytic cells. In this study, we focused on macrophage migration inhibitory factor (MIF) to evaluate its pathophysiological role in the phagocytic process. Immunohistochemical analysis of human pseudosynovial tissues retrieved at revision of total hip arthroplasty showed that infiltrating mononuclear and multinuclear cells were positively stained by both an anti‐CD68 antibody and anti‐human MIF antibody. For in vitro study, MIF was released from murine macrophage‐like cells (RAW 264.7) in response to phagocytosis of fluorescent‐latex beads in a particle dose‐dependent manner. Northern blot analysis showed marked elevation of the MIF mRNA level in the phagocytic macrophage‐like cells. Moreover, pretreatment of RAW 264.7 cells with rat recombinant MIF increased the extent of phagocytosis by 1·6‐fold compared with the control. Taken together, these results suggest that MIF plays an important role by activating macrophages in autocrine and paracrine fashion to phagocytose foreign particles.
ISSN:0019-2805
1365-2567
DOI:10.1046/j.1365-2567.1997.00311.x