Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family

Ogura cytoplasmic male sterility (CMS) in radish (Raphanus sativus) is caused by an aberrant mitochondrial gene, Orf138, that prevents the production of functional pollen without affecting female fertility. Rfo, a nuclear gene that restores male fertility, alters the expression of Orf138 at the post...

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Bibliographic Details
Published in:EMBO reports 2003-06, Vol.4 (6), p.588-594
Main Authors: Desloire, Sophie, Gherbi, Hassen, Laloui, Wassila, Marhadour, Sylvie, Clouet, Vanessa, Cattolico, Laurence, Falentin, Cyril, Giancola, Sandra, Renard, Michel, Budar, Françoise, Small, Ian, Caboche, Michel, Delourme, Régine, Bendahmane, Abdelhafid
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis thaliana
Cell Nucleus - metabolism
Chloroplasts - metabolism
Chromosome Mapping
Cloning, Molecular
Cytoplasm - metabolism
Databases as Topic
Genes, Plant
Genetic Markers
Life Sciences
Mitochondria - metabolism
Models, Genetic
Molecular Sequence Data
Phylogeny
Physical Chromosome Mapping
Plant Proteins - metabolism
Raphanus - genetics
Raphanus sativus
Scientific Report
Sequence Homology, Amino Acid
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Summary:Ogura cytoplasmic male sterility (CMS) in radish (Raphanus sativus) is caused by an aberrant mitochondrial gene, Orf138, that prevents the production of functional pollen without affecting female fertility. Rfo, a nuclear gene that restores male fertility, alters the expression of Orf138 at the post‐transcriptional level. The Ogura CMS/Rfo two‐component system is a useful model for investigating nuclear–cytoplasmic interactions, as well as the physiological basis of fertility restoration. Using a combination of positional cloning and microsynteny analysis of Arabidopsis thaliana and radish, we genetically and physically delimited the Rfo locus to a 15‐kb DNA segment. Analysis of this segment shows that Rfo is a member of the pentatricopeptide repeat (PPR) family. In Arabidopsis, this family contains more than 450 members of unknown function, although most of them are predicted to be targeted to mitochondria and chloroplasts and are thought to have roles in organellar gene expression.
ISSN:1469-221X
1469-3178
1469-221X
DOI:10.1038/sj.embor.embor848