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Imaging Cells and Tissues with Refractive Index Radiology
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enh...
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| Published in: | Biophysical journal 2004-12, Vol.87 (6), p.4180-4187 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c484t-cc4ea12154426ee3324d45de1b9c777ee8edc3739be2645fa483be707b73705b3 |
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| cites | cdi_FETCH-LOGICAL-c484t-cc4ea12154426ee3324d45de1b9c777ee8edc3739be2645fa483be707b73705b3 |
| container_end_page | 4187 |
| container_issue | 6 |
| container_start_page | 4180 |
| container_title | Biophysical journal |
| container_volume | 87 |
| creator | Hwu, Y. Tsai, W.L. Chang, H.M. Yeh, H.I. Hsu, P.C. Yang, Y.C. Su, Y.T. Tsai, H.L. Chow, G.M. Ho, P.C. Li, S.C. Moser, H.O. Yang, P. Seol, S.K. Kim, C.C. Je, J.H. Stefanekova, E. Groso, A. Margaritondo, G. |
| description | Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells. |
| doi_str_mv | 10.1529/biophysj.103.034991 |
| format | article |
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Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1529/biophysj.103.034991</identifier><identifier>PMID: 15465870</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cells ; Cells, Cultured - diagnostic imaging ; Cellular biology ; Humans ; Microscopy ; Radiographic Image Enhancement - methods ; Radiographic Image Interpretation, Computer-Assisted - methods ; Radiography - methods ; Radiology ; Refractometry - methods ; Spectroscopy, Imaging, Other Techniques ; Tissues</subject><ispartof>Biophysical journal, 2004-12, Vol.87 (6), p.4180-4187</ispartof><rights>2004 The Biophysical Society</rights><rights>Copyright Biophysical Society Dec 2004</rights><rights>Copyright © 2004, Biophysical Society 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-cc4ea12154426ee3324d45de1b9c777ee8edc3739be2645fa483be707b73705b3</citedby><cites>FETCH-LOGICAL-c484t-cc4ea12154426ee3324d45de1b9c777ee8edc3739be2645fa483be707b73705b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304927/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304927/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15465870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwu, Y.</creatorcontrib><creatorcontrib>Tsai, W.L.</creatorcontrib><creatorcontrib>Chang, H.M.</creatorcontrib><creatorcontrib>Yeh, H.I.</creatorcontrib><creatorcontrib>Hsu, P.C.</creatorcontrib><creatorcontrib>Yang, Y.C.</creatorcontrib><creatorcontrib>Su, Y.T.</creatorcontrib><creatorcontrib>Tsai, H.L.</creatorcontrib><creatorcontrib>Chow, G.M.</creatorcontrib><creatorcontrib>Ho, P.C.</creatorcontrib><creatorcontrib>Li, S.C.</creatorcontrib><creatorcontrib>Moser, H.O.</creatorcontrib><creatorcontrib>Yang, P.</creatorcontrib><creatorcontrib>Seol, S.K.</creatorcontrib><creatorcontrib>Kim, C.C.</creatorcontrib><creatorcontrib>Je, J.H.</creatorcontrib><creatorcontrib>Stefanekova, E.</creatorcontrib><creatorcontrib>Groso, A.</creatorcontrib><creatorcontrib>Margaritondo, G.</creatorcontrib><title>Imaging Cells and Tissues with Refractive Index Radiology</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.</description><subject>Animals</subject><subject>Cells</subject><subject>Cells, Cultured - diagnostic imaging</subject><subject>Cellular biology</subject><subject>Humans</subject><subject>Microscopy</subject><subject>Radiographic Image Enhancement - methods</subject><subject>Radiographic Image Interpretation, Computer-Assisted - methods</subject><subject>Radiography - methods</subject><subject>Radiology</subject><subject>Refractometry - methods</subject><subject>Spectroscopy, Imaging, Other Techniques</subject><subject>Tissues</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kVtLAzEQhYMoWi-_QJDFB9-2TjbZzeZBQYqXgiCIPodsdtqmbDc12Vb77420Xh98CmS-OTNnDiHHFPo0z-R5Zd18sgrTPgXWB8alpFukR3OepQBlsU16AFCksZDvkf0QpgA0y4Hukr0IFXkpoEfkcKbHth0nA2yakOi2Tp5sCAsMyavtJskjjrw2nV1iMmxrfEsedW1d48arQ7Iz0k3Ao817QJ5vrp8Gd-n9w-1wcHWfGl7yLjWGo6ZZnMizApGxjNc8r5FW0gghEEusDRNMVpgVPB9pXrIKBYhKMAF5xQ7I5Vp3vqhmkcW287pRc29n2q-U01b9rrR2osZuqSgDLjMRBc42At69RGOdmtlgol3dolsEVQjKZLxoBE__gFO38G00p-L-AqQUECG2hox3IXgcfW1CQX3koj5ziR9MrXOJXSc_TXz3bIKIwMUawHjKpUWvgrHYGqytR9Op2tl_B7wDfGagcQ</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Hwu, Y.</creator><creator>Tsai, W.L.</creator><creator>Chang, H.M.</creator><creator>Yeh, H.I.</creator><creator>Hsu, P.C.</creator><creator>Yang, Y.C.</creator><creator>Su, Y.T.</creator><creator>Tsai, H.L.</creator><creator>Chow, G.M.</creator><creator>Ho, P.C.</creator><creator>Li, S.C.</creator><creator>Moser, H.O.</creator><creator>Yang, P.</creator><creator>Seol, S.K.</creator><creator>Kim, C.C.</creator><creator>Je, J.H.</creator><creator>Stefanekova, E.</creator><creator>Groso, A.</creator><creator>Margaritondo, G.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20041201</creationdate><title>Imaging Cells and Tissues with Refractive Index Radiology</title><author>Hwu, Y. ; Tsai, W.L. ; Chang, H.M. ; Yeh, H.I. ; Hsu, P.C. ; Yang, Y.C. ; Su, Y.T. ; Tsai, H.L. ; Chow, G.M. ; Ho, P.C. ; Li, S.C. ; Moser, H.O. ; Yang, P. ; Seol, S.K. ; Kim, C.C. ; Je, J.H. ; Stefanekova, E. ; Groso, A. ; Margaritondo, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-cc4ea12154426ee3324d45de1b9c777ee8edc3739be2645fa483be707b73705b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cells</topic><topic>Cells, Cultured - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwu, Y.</au><au>Tsai, W.L.</au><au>Chang, H.M.</au><au>Yeh, H.I.</au><au>Hsu, P.C.</au><au>Yang, Y.C.</au><au>Su, Y.T.</au><au>Tsai, H.L.</au><au>Chow, G.M.</au><au>Ho, P.C.</au><au>Li, S.C.</au><au>Moser, H.O.</au><au>Yang, P.</au><au>Seol, S.K.</au><au>Kim, C.C.</au><au>Je, J.H.</au><au>Stefanekova, E.</au><au>Groso, A.</au><au>Margaritondo, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging Cells and Tissues with Refractive Index Radiology</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>87</volume><issue>6</issue><spage>4180</spage><epage>4187</epage><pages>4180-4187</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Address reprint requests to J. H. Je, E-mail: jhje@postech.ac.kr.</notes><abstract>Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15465870</pmid><doi>10.1529/biophysj.103.034991</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3495 |
| ispartof | Biophysical journal, 2004-12, Vol.87 (6), p.4180-4187 |
| issn | 0006-3495 1542-0086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1304927 |
| source | PubMed (Medline); BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
| subjects | Animals Cells Cells, Cultured - diagnostic imaging Cellular biology Humans Microscopy Radiographic Image Enhancement - methods Radiographic Image Interpretation, Computer-Assisted - methods Radiography - methods Radiology Refractometry - methods Spectroscopy, Imaging, Other Techniques Tissues |
| title | Imaging Cells and Tissues with Refractive Index Radiology |
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