Imaging Cells and Tissues with Refractive Index Radiology

Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enh...

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Bibliographic Details
Published in:Biophysical journal 2004-12, Vol.87 (6), p.4180-4187
Main Authors: Hwu, Y., Tsai, W.L., Chang, H.M., Yeh, H.I., Hsu, P.C., Yang, Y.C., Su, Y.T., Tsai, H.L., Chow, G.M., Ho, P.C., Li, S.C., Moser, H.O., Yang, P., Seol, S.K., Kim, C.C., Je, J.H., Stefanekova, E., Groso, A., Margaritondo, G.
Format: Article
Language:English
Subjects:
Animals
Cells
Cells, Cultured - diagnostic imaging
Cellular biology
Humans
Microscopy
Radiographic Image Enhancement - methods
Radiographic Image Interpretation, Computer-Assisted - methods
Radiography - methods
Radiology
Refractometry - methods
Spectroscopy, Imaging, Other Techniques
Tissues
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Summary:Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.103.034991