A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae

Meiotic recombination is not random along chromosomes; rather, there are preferred regions for initiation called hotspots. Although the general properties of meiotic hotspots are known, the requirements at the DNA sequence level for the determination of hotspot activity are still unclear. The sequen...

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Bibliographic Details
Published in:EMBO reports 2004-01, Vol.5 (1), p.41-46
Main Authors: Haring, Stuart J, Lautner, Lucas J, Comeron, Josep M, Malone, Robert E
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Base Sequence
Chromosome Breakage
Chromosomes, Fungal
Consensus Sequence
DNA, Fungal - genetics
Meiosis - genetics
Molecular Sequence Data
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Scientific Report
Sequence Deletion
Sequence Homology, Nucleic Acid
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Summary:Meiotic recombination is not random along chromosomes; rather, there are preferred regions for initiation called hotspots. Although the general properties of meiotic hotspots are known, the requirements at the DNA sequence level for the determination of hotspot activity are still unclear. The sequence of six known hotspots in Saccharomyces cerevisiae was compared to identify a common homology region (CoHR). They reported that the locations of CoHR sequences correspond to mapped double‐strand break (DSB) sites along three chromosomes (I, III, VI). We report here that a deletion of CoHR at HIS2, a hotspot used to identify the motif, has no significant effect on recombination. In the absence of CoHR, DSB formation occurs at a high frequency and at the same sequences as in wild‐type strains. In cases where the deletion of sequences containing the CoHR motif has been shown to reduce recombination, we propose that it may be a reflection of the location of the deletion, rather than the loss of CoHR, per se.
ISSN:1469-221X
1469-3178
1469-221X
DOI:10.1038/sj.embor.7400050