Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration

Agrobacterium tumefaciens causes crown gall disease in dicotyledonous plants by introducing a segment of DNA (T‐DNA), derived from its tumour‐inducing (Ti) plasmid, into plant cells at infection sites. Besides these natural hosts, Agrobacterium can deliver the T‐DNA also to monocotyledonous plants,...

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Bibliographic Details
Published in:The EMBO journal 2001-11, Vol.20 (22), p.6550-6558
Main Authors: van Attikum, Haico, Bundock, Paul, Hooykaas, Paul J. J.
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium tumefaciens
Agrobacterium tumefaciens - genetics
Antigens, Nuclear
Base Sequence
Chromosome Aberrations
DNA Helicases
DNA Ligase ATP
DNA Ligases - physiology
DNA, Bacterial - genetics
DNA, Bacterial - metabolism
DNA-Binding Proteins - physiology
DNA-Directed DNA Polymerase - metabolism
Electrophoresis, Polyacrylamide Gel
Endodeoxyribonucleases - physiology
Exodeoxyribonucleases - physiology
Fungal Proteins - physiology
Genetic Vectors
genomic instability
Genotype
Ku Autoantigen
Lig4 protein
Models, Genetic
Molecular Sequence Data
mre11 gene
Mre11 protein
Mutation
non-homologous end-joining
Nuclear Proteins - physiology
Polymerase Chain Reaction
Protein Binding
rad50 gene
Rad50 protein
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae
Sir4 protein
T-DNA integration
Telomere - metabolism
telomeres
xrs2 gene
Xrs2 protein
Yku70 protein
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Summary:Agrobacterium tumefaciens causes crown gall disease in dicotyledonous plants by introducing a segment of DNA (T‐DNA), derived from its tumour‐inducing (Ti) plasmid, into plant cells at infection sites. Besides these natural hosts, Agrobacterium can deliver the T‐DNA also to monocotyledonous plants, yeasts and fungi. The T‐DNA integrates randomly into one of the chromosomes of the eukaryotic host by an unknown process. Here, we have used the yeast Saccharomyces cerevisiae as a T‐DNA recipient to demonstrate that the non‐homologous end‐joining (NHEJ) proteins Yku70, Rad50, Mre11, Xrs2, Lig4 and Sir4 are required for the integration of T‐DNA into the host genome. We discovered a minor pathway for T‐DNA integration at the telomeric regions, which is still operational in the absence of Rad50, Mre11 or Xrs2, but not in the absence of Yku70. T‐DNA integration at the telomeric regions in the rad50, mre11 and xrs2 mutants was accompanied by gross chromosomal rearrangements.
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1093/emboj/20.22.6550