Signal transduction by epidermal growth factor and heregulin via the kinase-deficient ErbB3 protein

The role of protein tyrosine kinase activity in ErbB3-mediated signal transduction was investigated. ErbB3 was phosphorylated in vivo in response to either heregulin (HRG) in cells expressing both ErbB3 and ErbB2, or epidermal growth factor (EGF) in cells expressing both ErbB3 and EGF receptor. A re...

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Bibliographic Details
Published in:Biochemical journal 1998-08, Vol.334 ( Pt 1) (1), p.189-195
Main Authors: Kim, H H, Vijapurkar, U, Hellyer, N J, Bravo, D, Koland, J G
Format: Article
Language:English
Subjects:
3T3 Cells
Amino Acid Substitution
Animals
COS Cells
Epidermal Growth Factor - pharmacology
ErbB Receptors - biosynthesis
ErbB Receptors - genetics
ErbB Receptors - physiology
Glycoproteins - pharmacology
Mice
Mutagenesis, Site-Directed
Neuregulins
Phosphorylation
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - physiology
Receptor, ErbB-2 - biosynthesis
Receptor, ErbB-2 - genetics
Receptor, ErbB-2 - physiology
Receptor, ErbB-3
Recombinant Proteins - biosynthesis
Sequence Deletion
Signal Transduction - physiology
Transfection
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Summary:The role of protein tyrosine kinase activity in ErbB3-mediated signal transduction was investigated. ErbB3 was phosphorylated in vivo in response to either heregulin (HRG) in cells expressing both ErbB3 and ErbB2, or epidermal growth factor (EGF) in cells expressing both ErbB3 and EGF receptor. A recombinant receptor protein (ErbB3-K/M, in which K/M stands for Lys-->Met amino acid substitution) containing an inactivating mutation in the putative ATP-binding site was also phosphorylated in response to HRG and EGF. Both the wild-type ErbB3 and mutant ErbB3-K/M proteins transduced signals to phosphatidylinositol 3-kinase, Shc and mitogen-activated protein kinases. Separate kinase-inactivating mutations in the EGF receptor and ErbB2 proteins abolished ErbB3 phosphorylation and signal transduction activated by EGF and HRG respectively. Hence the protein tyrosine kinase activity necessary for growth factor signalling via the ErbB3 protein seems to be provided by coexpressed EGF and ErbB2 receptor proteins.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3340189