Human CPR (Cell Cycle Progression Restoration) Genes Impart a Far(-) Phenotype on Yeast Cells

Regulated cell cycle progression depends on the proper integration of growth control pathways with the basic cell cycle machinery. While many of the central molecules such as cyclins, CDKs, and CKIs are known, and many of the kinases and phosphatases that modify the CDKs have been identified, little...

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Bibliographic Details
Published in:Genetics (Austin) 1997-11, Vol.147 (3), p.1063-1076
Main Authors: Edwards, M. C, Liegeois, N, Horecka, J, DePinho, R. A, Sprague-Jr., G. F, Tyers, M, Elledge, S. J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cell Cycle Proteins
Cell Differentiation
Cell Division
Cellular biology
Chromosome Mapping
Congenital Abnormalities - genetics
Cyclin-Dependent Kinase Inhibitor Proteins
Cyclins - metabolism
Deoxyribonucleic acid
DNA
DNA, Complementary
DNA, Fungal
Fungal Proteins - genetics
Fungal Proteins - metabolism
G1 Phase
Gene Deletion
Genes, cdc
Genetics
Heat-Shock Proteins
HSP40 Heat-Shock Proteins
HSP90 Heat-Shock Proteins - metabolism
Humans
Investigations
Mating Factor
Membrane Glycoproteins
Molecular Chaperones - genetics
Molecular Sequence Data
Peptides - metabolism
Peptides - pharmacology
Phenotype
Pheromones - metabolism
Pheromones - pharmacology
Proteins
Repressor Proteins
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Signal Transduction
Transcription Factors - genetics
Yeast
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Summary:Regulated cell cycle progression depends on the proper integration of growth control pathways with the basic cell cycle machinery. While many of the central molecules such as cyclins, CDKs, and CKIs are known, and many of the kinases and phosphatases that modify the CDKs have been identified, little is known about the additional layers of regulation that impinge upon these molecules. To identify new regulators of cell proliferation, we have selected for human and yeast cDNAs that when overexpressed were capable of specifically overcoming G1 arrest signals from the cell cycle branch of the mating pheromone pathway, while still maintaining the integrity of the transcriptional induction branch. We have identified 13 human CPR (cell cycle progression restoration) genes and 11 yeast OPY (overproduction-induced pheromone-resistant yeast) genes that specifically block the G1 arrest by mating pheromone. The CPR genes represent a variety of biochemical functions including a new cyclin, a tumor suppressor binding protein, chaperones, transcription factors, translation factors, RNA-binding proteins, as well as novel proteins. Several CPR genes require individual CLNs to promote pheromone resistance and those that require CLN3 increase the basal levels of Cln3 protein. Moreover, several of the yeast OPY genes have overlapping functions with the human CPR genes, indicating a possible conservation of roles.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/147.3.1063