Repression of hybrid dysgenesis in Drosophila melanogaster by heat-shock-inducible sense and antisense P-element constructs

Sets of sense and antisense P-element constructs controlled by a heat-shock-inducible promoter were tested for their ability to repress manifestations of P-element activity in vivo. As a group, the antisense constructs repressed pupal lethality, a somatic manifestation of P activity, and this repres...

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Bibliographic Details
Published in:	Genetics (Austin) 1996-12, Vol.144 (4), p.1529-1544
Main Authors:	Simmons, M.J, Raymond, J.D, Grimes, C.D, Belinco, C, Haake, B.C, Jordan, M, Lund, C, Ojala, T.A, Papermaster, D
Format:	Article
Language:	eng
Subjects:	Animals antisense DNA antisense RNA beta-galactosidase Cellular biology DNA Transposable Elements - genetics Drosophila melanogaster Drosophila melanogaster - genetics dysgenesis enzyme activity Female gene expression Gene Expression Regulation genetic regulation Genetics gonadal dysgenesis heat shock proteins heat stress Heat Stress Disorders - genetics hsp70 promoter hybrids Insects Investigations Male mortality mutability mutation Oligonucleotides, Antisense - genetics promoter regions Promoter Regions, Genetic - genetics pupae pupal lethality recombinant DNA reporter genes transgenic animals transposons
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Summary:	Sets of sense and antisense P-element constructs controlled by a heat-shock-inducible promoter were tested for their ability to repress manifestations of P-element activity in vivo. As a group, the antisense constructs repressed pupal lethality, a somatic manifestation of P activity, and this repression was significantly enhanced by heat shock. Three of the 11 antisense constructs also repressed gonadal dysgenesis, a manifestation of P activity in the female germ line; however, none had any effect on P-element-mediated mutability in the male germ line. Among the 13 different heat-shock-inducible sense constructs that were tested, those containing the KP and DP elements were strong repressors of pupal lethality, gonadal dysgenesis and P-element-mediated mutability; however, individual lines carrying these constructs varied in their ability to repress each of these traits, presumably because of genomic position effects. With the exception of the sense construct that contained a complete P element, none of the sense or antisense constructs repressed a lacZ reporter gene driven by the P-element promoter. Overall, the experimental results suggest that in nature, P-element activity could be regulated by P-encoded polypeptides and by antisense P RNAs.
ISSN:	0016-6731 1943-2631 1943-2631