Germline transformation of Drosophila virilis mediated by the transposable element hobo

A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp 70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile G0 animal. Three transgenic insertions were cloned a...

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Bibliographic Details
Published in:Genetics (Austin) 1996, Vol.142 (1), p.173-177
Main Authors: Lozovskaya, E.R, Nurminsky, D.I, Hartl, D.L, Sullivan, D.T
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cloning, Molecular
DNA Nucleotidyltransferases - genetics
DNA Primers - genetics
DNA Transposable Elements
Drosophila - genetics
Drosophila virilis
Female
genetic transformation
Genetic Vectors
Genetics
germ cells
HSP70 Heat-Shock Proteins - genetics
In Situ Hybridization
Insects
insertion
Investigations
Male
Molecular Sequence Data
plasmid vectors
Plasmids - genetics
Repetitive Sequences, Nucleic Acid
Transformation, Genetic
Transposases
transposons
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Summary:A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp 70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile G0 animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known widespread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/142.1.173