Localization of the nic-7, ac-29 and thi-10 genes within the mating-type locus of Chlamydomonas reinhardtii

The tight linkage observed between the mating-type (mt) locus of Chlamydomonas reinhardtii and three auxotrophic mutations--nic-7 (nicotinamide-requiring), ac-29 (acetate-requiring), and thi-10 (thiamine-requiring)--has led to the hypothesis that recombination is suppressed in the mt region. The phy...

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Bibliographic Details
Published in:Genetics (Austin) 1995-10, Vol.141 (2), p.543-549
Main Author: Ferris, P.J. (Washington University, St. Louis, MO.)
Format: Article
Language:English
Subjects:
ACCOUPLEMENT
ACIDE ACETIQUE
ACIDO ACETICO
Animals
BESOIN NUTRITIONNEL
CHLAMYDOMONAS REINHARDTII
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - physiology
Chromosome Mapping
COPULA
Crosses, Genetic
DNA, Fungal - metabolism
DNA, Protozoan - metabolism
Freshwater
GENE
GENES
Genes, Plant
Genes, Protozoan
Genetic Linkage
GENETICA
Genetics
GENETIQUE
Investigations
LOCI
LOCUS
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Methylation
MUTACION
MUTATION
NECESIDADES DE NUTRIENTES
NICOTINAMIDA
NICOTINAMIDE
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
Restriction Mapping
SEGREGACION
SEGREGATION
Species Specificity
THIAMINE
TIAMINA
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transformation, Genetic
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Summary:The tight linkage observed between the mating-type (mt) locus of Chlamydomonas reinhardtii and three auxotrophic mutations--nic-7 (nicotinamide-requiring), ac-29 (acetate-requiring), and thi-10 (thiamine-requiring)--has led to the hypothesis that recombination is suppressed in the mt region. The physical location of these three genes has been established by transformation with sets of cloned DNA from the mt region. They lie to the left and right of the highly rearranged (R) domain of the mt locus, which has been proposed to be responsible for the recombinational suppression in the region. The cloned nic-7+ and thi-10+ genes will be useful as selectable markers for cotransformation experiments
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/141.2.543