Mutation in the bimD gene of Aspergillus nidulans confers a conditional mitotic block and sensitivity to DNA damaging agents

Mutation in the bimD gene of Aspergillus nidulans results in a mitotic block in anaphase by a defective mitosis. Mutation in bimD also confers, at temperatures permissive for the mitotic arrest phenotype, an increased sensitivity to DNA damaging agents, including methyl methanesulfonate and ultravio...

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Bibliographic Details
Published in:Genetics (Austin) 1993-08, Vol.134 (4), p.1085-1096
Main Authors: Denison, S.H, Kafer, E, May, G.S
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
ASPERGILLUS
ASPERGILLUS NIDULANS
Aspergillus nidulans - drug effects
Aspergillus nidulans - genetics
Base Sequence
Biological and medical sciences
BIOLOGICAL EFFECTS
CELL CYCLE
Cell Cycle Proteins
CELL DIVISION
Classical genetics, quantitative genetics, hybrids
CLONING
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA Damage
DNA HYBRIDIZATION
DNA, Fungal - drug effects
DNA-CLONING
ELECTROMAGNETIC RADIATION
EUMYCOTA
Fundamental and applied biological sciences. Psychology
Fungal Proteins - biosynthesis
Fungal Proteins - genetics
FUNGI
GENE
GENE MUTATIONS
GENES
Genes, Fungal
Genetics
Genetics of eukaryotes. Biological and molecular evolution
HYBRIDIZATION
INHIBITION
Investigations
Methyl Methanesulfonate - pharmacology
MITOSE
MITOSIS
Mitosis - genetics
Molecular Sequence Data
MUTACION INDUCIDA
MUTAGENE
MUTAGENOS
Mutagens - pharmacology
MUTANT
MUTANTES
Mutation
MUTATION PROVOQUEE
MUTATIONS
PLANTS
PROTEINAS
PROTEINE
Pteridophyta, spermatophyta
RADIACION ULTRAVIOLETA
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS 560130 > Radiation Effects on Microorganisms
RAYONNEMENT ULTRAVIOLET
Restriction Mapping
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
ULTRAVIOLET RADIATION
Ultraviolet Rays
Vegetals
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Summary:Mutation in the bimD gene of Aspergillus nidulans results in a mitotic block in anaphase by a defective mitosis. Mutation in bimD also confers, at temperatures permissive for the mitotic arrest phenotype, an increased sensitivity to DNA damaging agents, including methyl methanesulfonate and ultraviolet light. In order to better understand the relationship between DNA damage and mitotic progression, we cloned the bimD gene from Aspergillus. A cosmid containing the bimd gene was identified among pools of cosmids by cotransformation with the nutritional selective pyrG gene of a strain carrying the recessive, temperature-sensitive lethal bimD6 mutation. The bimD gene encodes a predicted polypeptide of 166,000 daltons in mass and contains amino acid sequence motifs similar to those found in some DNA-binding transcription factors. These sequences include a basic domain followed by a leucine zipper, which together are called a bZIP motif, and a carboxyl-terminal domain enriched in acidic amino acids. Overexpression of the wild-type bimD protein resulted in an arrest of the nuclear division cycle that was reversible and determined to be in either the G1 or S phase of the cell cycle. Our data suggest that bimD may play an essential regulatory role relating to DNA metabolism which is required for a successful mitosis
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/134.4.1085