Analysis of yeast retrotransposon Ty insertions at the CAN1 locus

The target site distribution for 55 independent Ty insertions that inactivate the function of the Saccharomyces cerevisiae CAN1 gene is reported. Under some selection conditions Ty elements inserted preferentially into the promoter and exhibited an orientation bias. In contrast, under other conditio...

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Bibliographic Details
Published in:Genetics (Austin) 1989-12, Vol.123 (4), p.655-665
Main Authors: Wilke, C.M. (University of Michigan, Ann Arbor, MI), Heidler, S.H, Brown, N, Liebman, S.W
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Canavanine - metabolism
Classical genetics, quantitative genetics, hybrids
CLONACION
CLONAGE
Cloning, Molecular
DNA Mutational Analysis
DNA Transposable Elements
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genetics of eukaryotes. Biological and molecular evolution
Investigations
PLASMIDE
PLASMIDIOS
Promoter Regions, Genetic
Recombination, Genetic
Restriction Mapping
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Thallophyta, bryophyta
Vegetals
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Summary:The target site distribution for 55 independent Ty insertions that inactivate the function of the Saccharomyces cerevisiae CAN1 gene is reported. Under some selection conditions Ty elements inserted preferentially into the promoter and exhibited an orientation bias. In contrast, under other conditions no insertions were detected in the promoter region and transposition appeared to occur randomly throughout the CAN1 coding sequence. These results show that the target site distribution for Ty insertions may be a function of the selection conditions
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/123.4.655