Cosuppression of Eukaryotic Release Factor 1-1 in Arabidopsis Affects Cell Elongation and Radial Cell Division

The role of the eukaryotic release factor 1 (eRF1) in translation termination has previously been established in yeast; however, only limited characterization has been performed on any plant homologs. Here, we demonstrate that cosuppression of eRF1-1 in Arabidopsis (Arabidopsis thaliana) has a profo...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2005-09, Vol.139 (1), p.115-126
Main Authors: Petsch, Katherine Anne, Mylne, Joshua, Botella, José Ramón
Format: Article
Language:English
Subjects:
Arabidopsis - cytology
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biological and medical sciences
Cell Biology and Signal Transduction
Cell Division
Cell Enlargement
Cell growth
Cell kinetics
Cell physiology
Down-Regulation
eukaryotic release factor
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
gene expression regulation
Gene Expression Regulation, Plant
Genes
Inflorescences
messenger RNA
molecular sequence data
nucleotide sequences
Peptide Termination Factors - deficiency
Peptide Termination Factors - genetics
Peptide Termination Factors - metabolism
Phenotype
Phenotypes
phenotypic variation
plant biochemistry
plant genetics
Plant Leaves - anatomy & histology
Plant Leaves - genetics
plant morphology
plant physiology
Plant physiology and development
plant proteins
Plant Stems - metabolism
plant vascular system
Plants
Plants, Genetically Modified
RNA
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Plant - genetics
RNA, Plant - metabolism
Transgenes
Transgenic plants
Yeasts
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Summary:The role of the eukaryotic release factor 1 (eRF1) in translation termination has previously been established in yeast; however, only limited characterization has been performed on any plant homologs. Here, we demonstrate that cosuppression of eRF1-1 in Arabidopsis (Arabidopsis thaliana) has a profound effect on plant morphology, resulting in what we term the broomhead phenotype. These plants primarily exhibit a reduction in internode elongation causing the formation of a broomhead-like cluster of malformed siliques at the top of the inflorescence stem. Histological analysis of broomhead stems revealed that cells are reduced in height and display ectopic lignification of the phloem cap cells, some phloem sieve cells, and regions of the fascicular cambium, as well as enhanced lignification of the interfascicular fibers. We also show that cell division in the fascicular cambial regions is altered, with the majority of vascular bundles containing cambial cells that are disorganized and possess enlarged nuclei. This is the first attempt at functional characterization of a release factor in vivo in plants and demonstrates the importance of eRF1-1 function in Arabidopsis.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.105.062695