Aphid transmission of cauliflower mosaic virus requires the viral PIII protein

The open reading frame (ORF) III product (PIII) of cauliflower mosaic virus is necessary for the infection cycle but its role is poorly understood. We have used in vitro protein binding (‘far Western’) assays to demonstrate that PIII interacts with the cauliflower mosaic virus (CaMV) ORF II product...

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Bibliographic Details
Published in:The EMBO journal 1999-12, Vol.18 (24), p.7077-7085
Main Authors: Leh, V, Jacquot, E, Geldreich, A, Hermann, T, Leclerc, D, Cerutti, M, Yot, P, Keller, M, Blanc, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
aphid transmisison protein
aphid transmission factor
Aphididae
Aphids - virology
Base Sequence
binding proteins
binding sites
Brassica - virology
Brassica rapa subsp. rapa
Capsid - genetics
Capsid - metabolism
Capsid Proteins
Cauliflower mosaic virus
Caulimovirus - genetics
Caulimovirus - physiology
Cellular Biology
Cloning, Molecular
Defective Viruses - genetics
Defective Viruses - physiology
deletions
disease transmission
DNA Primers
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Escherichia coli
far Western
Genetic Complementation Test
Life Sciences
Models, Molecular
molecular conformation
Molecular Sequence Data
mutagenesis
Mutagenesis, Site-Directed
mutation
Myzus persicae
Open Reading Frames
ORF III product
PII protein
PIII protein
Point Mutation
Polymerase Chain Reaction
protein secondary structure
Protein Structure, Secondary
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Sequence Deletion
transmission
Viral Fusion Proteins - chemistry
Viral Fusion Proteins - genetics
Viral Fusion Proteins - metabolism
viral proteins
Virion - genetics
Virion - physiology
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Description
Summary:The open reading frame (ORF) III product (PIII) of cauliflower mosaic virus is necessary for the infection cycle but its role is poorly understood. We have used in vitro protein binding (‘far Western’) assays to demonstrate that PIII interacts with the cauliflower mosaic virus (CaMV) ORF II product (PII), a known aphid transmission factor. Aphid transmission of purified virions of the PII‐defective strain CM4‐184 was dependent upon added PII, but complementation was efficient only in the presence of PIII, demonstrating the requirement of PIII for transmission. Deletion mutagenesis mapped the interaction domains of PIII and PII to the 30 N‐terminal and 61 C‐terminal residues of PIII and PII, respectively. A model for interaction between PIII and PII is proposed on the basis of secondary structure predictions. Finally, a direct correlation between the ability of PIII and PII to interact and aphid transmissibility of the virus was demonstrated by using mutagenized PIII proteins. Taken together, these data argue strongly that PIII is a second ‘helper’ factor required for CaMV transmission by aphids.
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1093/emboj/18.24.7077