Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion

SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole fusion are combined in the six‐subunit HOPS complex. HOPS facilitates Ypt7p nucleotide exchange, is a Ypt7p effector, and contains an SM pro...

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Bibliographic Details
Published in:The EMBO journal 2005-05, Vol.24 (10), p.1775-1786
Main Authors: Collins, Kevin M, Thorngren, Naomi L, Fratti, Rutilio A, Wickner, William T
Format: Article
Language:English
Subjects:
Carrier Proteins - metabolism
fusion
Genes, Reporter
HOPS
membrane
Membrane Proteins - metabolism
Rab
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
SNARE
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Vacuoles - metabolism
Vesicular Transport Proteins - metabolism
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Summary:SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole fusion are combined in the six‐subunit HOPS complex. HOPS facilitates Ypt7p nucleotide exchange, is a Ypt7p effector, and contains an SM protein. We have dissected the associations and requirements for HOPS, Ypt7p, and Sec17/18p during SNARE complex assembly. Vacuole SNARE complexes bind either Sec17p or the HOPS complex, but not both. Sec17p and its co‐chaperone Sec18p disassemble SNARE complexes. Ypt7p regulates the reassembly of unpaired SNAREs with each other and with HOPS, forming HOPS·SNARE complexes prior to fusion. After HOPS·SNARE assembly, lipid rearrangements are still required for vacuole content mixing. Thus, Sec17p and HOPS have mutually exclusive interactions with vacuole SNAREs to mediate disruption of SNARE complexes or their assembly for docking and fusion. Sec17p may displace HOPS from SNAREs to permit subsequent rounds of fusion.
ISSN:0261-4189
1460-2075
DOI:10.1038/sj.emboj.7600658