High-resolution plasma metabolomics and thiamine status in critically Ill adult patients

Introduction Thiamine (Vitamin B1) is an essential micronutrient and is classically considered a co-factor in energy metabolism. The association between thiamine status and whole-body metabolism in critical illness has not been studied. Objectives To determine association between whole blood thiamin...

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Published in:Metabolomics 2024-07, Vol.20 (4), p.83, Article 83
Main Authors: Gundogan, Kursat, Nellis, Mary M., Ozer, Nurhayat T., Ergul, Serap S., Sahin, Gulsah G., Temel, Sahin, Yuksel, Recep C., Teeny, Sami, Alvarez, Jessica A., Sungur, Murat, Jones, Dean P., Ziegler, Thomas R.
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Biomedical and Life Sciences
Biomedicine
Blood levels
Carbohydrates
Cell Biology
Developmental Biology
Energy metabolism
Furosemide
Intestinal microflora
Life Sciences
Lipid metabolism
Liquid chromatography
Mannose
Mass spectroscopy
Metabolic pathways
Metabolism
Metabolites
Metabolomics
Microbiomes
Molecular Medicine
Original
Original Article
Pentose phosphate pathway
Plasma
Thiamine
Vitamin B
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Summary:Introduction Thiamine (Vitamin B1) is an essential micronutrient and is classically considered a co-factor in energy metabolism. The association between thiamine status and whole-body metabolism in critical illness has not been studied. Objectives To determine association between whole blood thiamine pyrophosphate (TPP) concentrations and plasma metabolites and connected metabolic pathways using high resolution metabolomics (HRM) in critically ill patients. Methods Cross-sectional study performed at Erciyes University Hospital, Kayseri, Turkey and Emory University, Atlanta, GA, USA. Participants were critically ill adults with an expected length of intensive care unit stay longer than 48 h and receiving chronic furosemide therapy. A total of 76 participants were included. Mean age was 69 years (range 33–92 years); 65% were female. Blood for TPP and metabolomics was obtained on the day of ICU admission. Whole blood TPP was measured by HPLC and plasma HRM was performed using liquid chromatography/mass spectrometry. Data was analyzed using regression analysis of TPP levels against all plasma metabolomic features in metabolome-wide association studies (MWAS). MWAS using the highest and lowest TPP concentration tertiles was performed as a secondary analysis. Results Specific metabolic pathways associated with whole blood TPP levels in regression and tertile analysis included pentose phosphate, fructose and mannose, branched chain amino acid, arginine and proline, linoleate, and butanoate pathways. Conclusions Plasma HRM revealed that thiamine status, determined by whole blood TPP concentrations, was significantly associated with metabolites and metabolic pathways related to metabolism of energy, carbohydrates, amino acids, lipids, and the gut microbiome in adult critically ill patients.
ISSN:1573-3890
1573-3882
1573-3890
DOI:10.1007/s11306-024-02144-9