A new standardization for the use of chicken embryo: selection of target from the phage display library and infection

The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic...

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Published in:Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.412, Article 412
Main Authors: de Souza, Jessica Brito, Sommerfeld, Simone, Almeida-Souza, Hebréia Oliveira, Vaz, Emília Rezende, Bastos, Luciana Machado, Santos, Fabiana de Almeida Araújo, Rodrigues, Alessandra Castro, Medeiros-Ronchi, Alessandra Aparecida, Goulart, Luiz Ricardo, Fonseca, Belchiolina Beatriz
Format: Article
Language:English
Subjects:
Applied Microbial and Cell Physiology
Bacteria
Biomedical and Life Sciences
Biotechnology
Chickens
Disseminated infection
E coli
Embryos
Food industry
Immune response
Infections
Injury analysis
Libraries
Life Sciences
Microbial Genetics and Genomics
Microbiology
Microorganisms
Peptides
Phage display
Phages
Poultry
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Summary:The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic microorganisms, such as bacteria and viruses. This study evaluated the use of phage M13 in the chicken embryos model. We inoculated 13-day-old chicken embryos with Salmonella Pullorum (SP) and then evaluated survival for the presence of phage M13 or E. coli ER2738 (ECR) infected with M13. We found that the ECR bacterium inhibits SP multiplication in 0.32 (M13-infected ECR) or 0.44 log UFC/mL (M13-uninfected ECR) and that the ECR-free phage M13 from the PD library can be used in chicken embryo models. This work provides the use of the chicken embryo as a model to study systemic infection and can be employed as an analysis tool for various peptides that M13 can express from PD selection. Key points • SP-infected chicken embryo can be a helpful model of systemic infection for different tests. • Phage M13 does not lead to embryonic mortality or cause serious injury to embryos. • Phage M13 from the PD library can be used in chicken embryo model tests.
ISSN:0175-7598
1432-0614
1432-0614
DOI:10.1007/s00253-024-13227-x