3D bioprinted mesenchymal stem cell laden scaffold enhances subcutaneous vascularization for delivery of cell therapy

Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment of various diseases. However, the subdermal site is poorly vascularized making it inadequate for supporting engraftment, viability, and function of exogenous cells. In this study, we developed a 3D bi...

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Bibliographic Details
Published in:Biomedical microdevices 2024-09, Vol.26 (3), p.29
Main Authors: Bo, Tommaso, Pascucci, Elia, Capuani, Simone, Campa-Carranza, Jocelyn Nikita, Franco, Letizia, Farina, Marco, Secco, Jacopo, Becchi, Sara, Cavazzana, Rosanna, Joubert, Ashley L., Hernandez, Nathanael, Chua, Corrine Ying Xuan, Grattoni, Alessandro
Format: Article
Language:English
Subjects:
3-D printers
Alginates
Alginic acid
Alzheimer's disease
Angiogenesis
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biophysics
Blood vessels
Cell therapy
Crosslinking
Cytokines
Disease
Engineering
Engineering Fluid Dynamics
Extracellular matrix
Gelatin
Hydrogels
Ischemia
Mechanical properties
Mesenchymal stem cells
Metabolism
Microenvironments
Nanotechnology
Polyethylene glycol
Scaffolds
Stem cells
Three dimensional printing
Tissues
Vascular endothelial growth factor
Vascular tissue
Vascularization
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Summary:Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment of various diseases. However, the subdermal site is poorly vascularized making it inadequate for supporting engraftment, viability, and function of exogenous cells. In this study, we developed a 3D bioprinted scaffold composed of alginate/gelatin (Alg/Gel) embedded with mesenchymal stem cells (MSCs) to enhance vascularization and tissue ingrowth in a subcutaneous microenvironment. We identified bio-ink crosslinking conditions that optimally recapitulated the mechanical properties of subcutaneous tissue. We achieved controlled degradation of the Alg/Gel scaffold synchronous with host tissue ingrowth and remodeling. Further, in a rat model, the Alg/Gel scaffold was superior to MSC-embedded Pluronic hydrogel in supporting tissue development and vascularization of a subcutaneous site. While the scaffold alone promoted vascular tissue formation, the inclusion of MSCs in the bio-ink further enhanced angiogenesis. Our findings highlight the use of simple cell-laden degradable bioprinted structures to generate a supportive microenvironment for cell delivery. Graphical Abstract
ISSN:1387-2176
1572-8781
1572-8781
DOI:10.1007/s10544-024-00713-2