Effects of glucagon-like peptide-1 receptor agonists on spermatogenesis-related gene expression in mouse testis and testis-derived cell lines

Glucagon-like peptide-1 (GLP-1) is an incretin released into the gastrointestinal tract after food ingestion, and stimulates insulin secretion from the beta cells of the pancreatic islets. Incretins have recently been reported to have extrapancreatic actions, and they are anticipated to have potenti...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Veterinary Medical Science 2024, Vol.86(5), pp.555-562
Main Authors: IIDA, Masashi, ASANO, Atsushi
Format: Article
Language:English
Subjects:
Adipose tissue
Agonists
Animals
Beta cells
bucladesine
Cell Line
Cell lines
Diabetes mellitus
exenatide
Exenatide - pharmacology
Gastrointestinal tract
Gene expression
Gene Expression - drug effects
Gene Expression Regulation - drug effects
GLP-1 protein
Glucagon
Glucagon-like peptide 1
Glucagon-Like Peptide 1 - metabolism
Glucagon-Like Peptide 1 - pharmacology
Glucagon-like peptide-1 receptor
Glucagon-Like Peptide-1 Receptor - genetics
Glucagon-Like Peptide-1 Receptor - metabolism
Glucagon-Like Peptide-1 Receptor Agonists
Infertility
Insulin secretion
Laboratory Animal Science
Leydig Cells - drug effects
Leydig Cells - metabolism
Male
Mice
Peptides
Peptides - pharmacology
Sertoli cell
Sertoli cells
Sertoli Cells - drug effects
Sertoli Cells - metabolism
Spermatogenesis
Spermatogenesis - drug effects
Testes
Testis - drug effects
Testis - metabolism
Venoms - pharmacology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glucagon-like peptide-1 (GLP-1) is an incretin released into the gastrointestinal tract after food ingestion, and stimulates insulin secretion from the beta cells of the pancreatic islets. Incretins have recently been reported to have extrapancreatic actions, and they are anticipated to have potential efficacy for conditions such as male infertility as well as diabetes. However, the effects of incretins on male reproductive function remain unclear. In this study, GLP-1 receptor expression and the effects of GLP-1 on spermatogenesis-associated genes were investigated using mouse testes and testis-derived cultured cell lines. Glp1r mRNA and GLP-1 protein were expressed in mouse testes at levels comparable to or greater than those in positive control adipose tissue, and the liver and intestine, and also in a Sertoli cell line (TM4) and a Leydig cell line (MA-10) as well as the GC-1 spg and GC-2 spd (ts) germ cell lines. TM4 cells treated with the GLP-1 receptor agonist exenatide showed transiently and significantly upregulated Kitl, Pdgfa, and Glp1r mRNA expression. Furthermore, at 1 hr post-exenatide administration to male mice, Kitl and Glp1r mRNA expression levels were significantly increased, and Pdgfa mRNA expression level also showed a tendency toward increase. TM4 cells were treated with various cell-activating agents, and bucladesine elicited significantly increased Glp1r mRNA expression. We suggest that GLP-1 provides acute stimulation of Sertoli cells in the mouse testis and has a stimulatory effect on the expression of spermatogenesis-related genes.
ISSN:0916-7250
1347-7439
1347-7439
DOI:10.1292/jvms.24-0042